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Restriction Endonuclease Cleavage Maps of Animal Mitochondrial DNAs

Brown, Wesley M. and Vinograd, Jerome (1974) Restriction Endonuclease Cleavage Maps of Animal Mitochondrial DNAs. Proceedings of the National Academy of Sciences of the United States of America, 71 (11). pp. 4617-4621. ISSN 0027-8424.

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The restriction endonuclease, HindIII, gives rise to three fragments in each of the three mitochondrial DNAs isolated from the established mammalian cell lines LA9 (mouse), HeLa (human), and BSC-1 (African green monkey). The restriction endonuclease, EcoRI, gives rise to three fragments in mitochondrial DNA from HeLa and to two in DNAs from LA9 and BSC-1. The sizes and the orders of the fragments in the respective genomes have been determined with data obtained from the electron microscope. The origin and the direction of replication have been designated in each of the cleavage maps. Polyacrylamide gel electrophoretic analyses demonstrated that additional fragments not detectable in the electron microscope and larger than 50 nucleotide pairs were not present.

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Additional Information:© 1974 National Academy of Sciences. Contributed by Jerome Vinograd, September 3, 1974. We thank H. W. Boyer, University of California, San Francisco, and P. H. Johnson for generously providing enzymes, Denzille Stephens for assistance in cell culture, and Frank Mayer for assistance in measuring molecules. We also thank D. Clayton and D. Robberson for informing us of their preliminary results on the EcoRI maps of mouse and human mtDNAs. This work was supported in part by USPHS NIH Grants GM15327 and CA08014 and by a National Science Foundation Training Grant to W.M.B. This is contribution no. 4962 from the Division of Chemistry and Chemical Engineering.
Funding AgencyGrant Number
Public Health ServiceGM15327
Public Health ServiceCA08014
Subject Keywords:electron microscopy; replication; human, monkey, and mouse mitochondrial DNAs
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Caltech Division of Chemistry and Chemical Engineering4962
Issue or Number:11
Record Number:CaltechAUTHORS:20120724-161806865
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:32693
Deposited On:25 Jul 2012 14:18
Last Modified:03 Oct 2019 04:03

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