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Motility of triton-demembranated sea urchin sperm flagella during digestion by trypsin

Brokaw, C. J. and Simonick, T. F. (1977) Motility of triton-demembranated sea urchin sperm flagella during digestion by trypsin. Journal of Cell Biology, 75 (3). pp. 650-665. ISSN 0021-9525. PMCID PMC2111580. doi:10.1083/jcb.75.3.650.

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The survival curves for a population of reactivated spermatozoa exposed to digestion by trypsin indicate that a large number of trypsin-sensitive targets must be digested before the flagellum disintegrates. Changes in flagellar movement during trypsin digestion can be very small, especially when the spermatozoa are reactivated at 0.25 M KCl. They are not the changes which would be expected if elastic resistance of the trypsin-sensitive structures responsible for maintaining the integrity of the axoneme is a significant determinant of flagellar bend amplitude. By carrying out trypsin digestion under a variety of conditions, at least six distinct effects of trypsin digestion on parameters of flagellar movement have been detected. These include a gradual increase in the rate of sliding between tubules, gradual and abrupt changes in beat frequency accompanied by reciprocal decreases in bend angle, changes in the symmetry and planarity of bending, and selective interference with mechanisms for bend initiation and bend propagation.

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Additional Information:© 1977 The Rockefeller University Press. Received for publication 27 May 1977, and in revised form 1 August 1977. We thank Eileen Roy for assisting with these experiments. We appreciate the support of the U. S. Public Health Service through a National Institutes of Health grant GM 18711.
Funding AgencyGrant Number
NIHGM 18711
Subject Keywords:flagella; motility; trypsin; biological oscillation; microtubule
Issue or Number:3
PubMed Central ID:PMC2111580
Record Number:CaltechAUTHORS:20120725-095727895
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:32706
Deposited On:25 Jul 2012 17:46
Last Modified:09 Nov 2021 21:29

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