A Caltech Library Service

Morphogenesis of Poliovirus II. Demonstration of a New Intermediate, the Proviron

Fernandez-Tomas, Carlos B. and Baltimore, David (1973) Morphogenesis of Poliovirus II. Demonstration of a New Intermediate, the Proviron. Journal of Virology, 12 (5). pp. 1122-1130. ISSN 0022-538X. PMCID PMC356744.

PDF - Published Version
See Usage Policy.


Use this Persistent URL to link to this item:


Poliovirus-infected cells contain a previously unrecognized particle which appears to be an intermediate in virion synthesis and therefore has been named proviron. It sediments at about 125S, contains the three procapsid proteins, VP-0, VP-1, and VP-3, and has 35S viral RNA. It is disrupted both by sodium dodecyl sulfate and EDTA but the RNA resists digestion by ribonuclease. Pulsechase experiments and studies employing the virus-specific inhibitor, guanidine, all indicate that the proviron is formed by combination of newly made RNA with the procapsid. Cleavage of VP-0 to form VP-2 and VP-4 follows formation of the provirion and would be the final step in poliovirus morphogenesis.

Item Type:Article
Related URLs:
URLURL TypeDescription CentralArticle
Baltimore, David0000-0001-8723-8190
Additional Information:© 1973 American Society for Microbiology. Received for publication 21 June 1973. We thank Donna Smoler for expert technical assistance and Alice Huang and Charles Cole for critical reading of the manuscript. This investigation was supported by Public Health Service grant AI-08388 from The National Institute of Allergy and Infectious Diseases. C.B.F. was a post-doctoral fellow of the Damon Runyon Memorial Fund for Cancer Research and D.B. was an American Cancer Society research professor.
Funding AgencyGrant Number
Damon Runyan Memorial Fund for Cancer ResearchUNSPECIFIED
American Cancer SocietyUNSPECIFIED
Issue or Number:5
PubMed Central ID:PMC356744
Record Number:CaltechAUTHORS:20120726-100027537
Persistent URL:
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:32743
Deposited On:26 Jul 2012 21:54
Last Modified:03 Oct 2019 04:03

Repository Staff Only: item control page