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Location of the 5-Methylcytosine Group on the Bacteriophage φX174 Genome

Lee, Amy Shiu and Sinsheimer, Robert L. (1974) Location of the 5-Methylcytosine Group on the Bacteriophage φX174 Genome. Journal of Virology, 14 (4). pp. 872-877. ISSN 0022-538X. PMCID PMC355593.

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Bacteriophage φX174 DNA was labeled in vivo with [methyl-^(3)H]methionine. The methyl-labeled progeny DNA was extracted from purified bacteriophage φX174 particles and was used as template for in vitro synthesis of the complementary strand in the presence of the nucleoside triphosphates and Escherichia coli polymerase I. The resultant replicative form DNA was then cleaved, in separate experiments, with restriction endonucleases from Haemophilus influenzae and H. aegyptius. The DNA fragments were analyzed by polyacrylamide gel electrophoresis. It is concluded that the single methylcytosine in the viral DNA is located in a specific region of the φX174 genome, very likely in gene H.

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Additional Information:© 1974 American Society for Microbiology. Received for publication 6 June 1974. This work was supported by Public Health Service Grant GM13554 from the National Institute of General Medical Sciences.
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Issue or Number:4
PubMed Central ID:PMC355593
Record Number:CaltechAUTHORS:20120726-100159402
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:32744
Deposited On:30 Jul 2012 16:09
Last Modified:03 Oct 2019 04:03

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