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Defective Interfering Particles of Poliovirus I. Isolation and Physical Properties

Cole, Charles N. and Smoler, Donna and Wimmer, Eckard and Baltimore, David (1971) Defective Interfering Particles of Poliovirus I. Isolation and Physical Properties. Journal of Virology, 7 (4). pp. 478-485. ISSN 0022-538X. PMCID PMC356147.

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A class of defective interfering (DI) poliovirus particles has been identified. The first was found as a contaminant of a viral stock; others have been isolated by serial passage at a high multiplicity of infection. The DI particles are less dense than standard virus and sediment more slowly. Their ribonucleic acid (RNA) sediments more slowly than standard RNA and has a higher electrophoretic mobility. Competition hybridization experiments with double-stranded viral RNA indicate that DI RNA is 80 to 90% of the length of standard RNA. The proteins of DI particles are indistinguishable from those of standard poliovirus.

Item Type:Article
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URLURL TypeDescription CentralArticle
Baltimore, David0000-0001-8723-8190
Additional Information:© 1971 American Society for Microbiology. Received for publication 8 January 1971. We would like to thank David Rekosh for his participation in the accident leading to the initial discovery of poliovirus DI, and Alice S. Huang for critical reading of the manuscript. This work was supported by Public Health Service grant Al-08388 from the National Institute of Allergy and Infectious Diseases. C. N. C. was a predoctoral fellow of the National Science Foundation and D. B. was a Faculty Research Awardee of the American Cancer Society.
Funding AgencyGrant Number
NIHAl- 08388
NSF Predoctoral FellowshipUNSPECIFIED
American Cancer SocietyUNSPECIFIED
Issue or Number:4
PubMed Central ID:PMC356147
Record Number:CaltechAUTHORS:20120726-114113130
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:32748
Deposited On:26 Jul 2012 21:45
Last Modified:03 Oct 2019 04:03

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