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Relationship of retrovirus polyprotein cleavages to virion maturation studied with temperature-sensitive murine leukemia virus mutants

Witte, Owen N. and Baltimore, David (1978) Relationship of retrovirus polyprotein cleavages to virion maturation studied with temperature-sensitive murine leukemia virus mutants. Journal of Virology, 26 (3). pp. 750-761. ISSN 0022-538X. PMCID PMC525900. https://resolver.caltech.edu/CaltechAUTHORS:20120726-131556638

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Abstract

Murine leukemia virus mutants ts3 (Moloney) and ts24 (Rauscher) both formed late-budding structures on the cell membrane at restrictive temperature. They both accumulated core polyproteins Pr65gag and Pr180gag-pol in cell membranes, but the envelope precursor was rapidly turned over. After shift to permissive temperature in the presence of cycloheximide, the accumulated precursors were sequentially cleaved via discrete intermediates both during the final stages of the budding process and in newly released virions to yield the finished virion core proteins and reverse transcriptase. The precursor form of reverse transcriptase was not enzymatically active and became activated partially or entirely inside released virions.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://jvi.asm.org/content/26/3/750.abstractPublisherArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC525900/PubMed CentralArticle
ORCID:
AuthorORCID
Witte, Owen N.0000-0003-4461-4533
Baltimore, David0000-0001-8723-8190
Additional Information:© 1978 American Society for Microbiology. Received for publication 3 January 1978. We thank Claire Moore for performing the electron microscopy and Michael Paskind for excellent photographic work. This research was supported by American Cancer Society grant VC-41, Public Health Service grant CA-14051 from the National Cancer Institute, and a contract from the Virus Cancer Program of the National Cancer Institute. O. N. W. is a Helen Hay Whitney Foundation postdoctoral fellow. D. B. is an American Cancer Society Professor of Microbiology.
Funders:
Funding AgencyGrant Number
American Cancer SocietyVC-41
NIHCA-14051
National Cancer InstituteUNSPECIFIED
Helen Hay Whitney FoundationUNSPECIFIED
Issue or Number:3
PubMed Central ID:PMC525900
Record Number:CaltechAUTHORS:20120726-131556638
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20120726-131556638
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:32754
Collection:CaltechAUTHORS
Deposited By: Jason Perez
Deposited On:30 Jul 2012 16:04
Last Modified:03 Oct 2019 04:03

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