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Preparation of Syngeneic Tumor Regressor Serum Reactive with the Unique Determinants of the Abelson Murine Leukemia Virus-Encoded P120 Protein at the Cell Surface

Witte, O. N. and Rosenberg, N. and Baltimore, D. (1979) Preparation of Syngeneic Tumor Regressor Serum Reactive with the Unique Determinants of the Abelson Murine Leukemia Virus-Encoded P120 Protein at the Cell Surface. Journal of Virology, 31 (3). pp. 776-784. ISSN 0022-538X. PMCID PMC353505.

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Antisera reactive with the Abelson murine leukemia virus (A-MuLV)-specified P120 (anti-AbT sera) were produced in C57L/J mice. Of many strains tested, only C57L/J reproducibly rejected syngenic A-MuLV-induced tumor cells; after multiple immunizations their sera would immunoprecipitate both P120 and Moloney-MuLV (M-MuLV) proteins. Using labeled A-MuLV-induced nonproducer cells, only P120 could be detected by anti-AbT sera, suggesting that it may be the only A-MuLV-specified protein. Reactivity of anti-AbT sera with P120 was not blocked by M-MuLV virion proteins, implying that the sera recognize a portion of P120 that is not homologous to any M-MuLV product. Anti-AbT sera stained the surface of live, A-MuLV-transformed nonproducer cells in a two-stage immunofluorescence assay, and such staining was not blocked by M-MuLV protein. Also, intact A-MuLV-transformed cells absorbed much of the reactivity of certain anti-AbT sera for P120. Thus a portion of P120 appears to be exposed on the surface of transformed cells. P120 lacks detectable carbohydrate, is not affected by endoglycosidase H, and cannot be labeled by lactoperoxidase-catalyzed iodination. Thus P120 is an unusual surface protein.

Item Type:Article
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URLURL TypeDescription CentralArticle
Witte, O. N.0000-0003-4461-4533
Baltimore, D.0000-0001-8723-8190
Additional Information:© 1979 American Society for Microbiology. Received for publication 30 March 1979. We gratefully acknowledge the superb technical assistance of Dan Clark and Leslie Sun. This work was supported by grant VC-4J from the American Cancer Society and Public Health Service grants CA-14051 and CA-24220 from the National Cancer Institute. O.N.W. is a Helen Hay Whitney Postdoctoral Fellow. N.R. is a Research Scholar of the American Cancer Society, Massachusetts Division. D.B. is a Research Professor of the American Cancer Society.
Funding AgencyGrant Number
American Cancer SocietyVC-4J
Helen Hay Whitney FoundationUNSPECIFIED
American Cancer Society, Massachusetts DivisionUNSPECIFIED
Issue or Number:3
PubMed Central ID:PMC353505
Record Number:CaltechAUTHORS:20120727-131617278
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Official Citation:Preparation of syngeneic tumor regressor serum reactive with the unique determinants of the Abelson murine leukemia virus-encoded P120 protein at the cell surface. O N Witte, N Rosenberg, and D Baltimore J. Virol. September 1979 31:776-784
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:32771
Deposited On:30 Jul 2012 15:13
Last Modified:03 Oct 2019 04:03

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