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Mesh-supported submicron parylene-C membranes for culturing retinal pigment epithelial cells

Lu, Bo and Zhu, Danhong and Hinton, David and Humayun, Mark S. and Tai, Yu-Chong (2012) Mesh-supported submicron parylene-C membranes for culturing retinal pigment epithelial cells. Biomedical Microdevices, 14 (4). pp. 659-667. ISSN 1387-2176. https://resolver.caltech.edu/CaltechAUTHORS:20120730-114002078

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Abstract

In this work, a mesh-supported submicron parylene- C membrane (MSPM) is proposed as an artificial Bruch’s membrane for the therapy of age-related macular degeneration (AMD). Any artificial Bruch’s membrane must first satisfy two important requirements. First, it should be as permeable as healthy human Bruch’s membrane to support nutrients transportation. Secondly, it should be able to support the adherence and proliferation of retinal pigment epithelial (RPE) cells with in vivo-like morphologies and functions. Although parylene-C is widely used as a barrier layer in many biomedical applications, it is found that parylene-C membranes with submicron thickness are semipermeable to macromolecules.We first measure the permeability of submicron parylene-C and find that 0.15–0.30 μm parylene-C has similar permeability to healthy human Bruch’s membranes. Blind-well perfusion cell viability experiments further demonstrate that nutrients and macromolecules can diffuse across 0.30 μm parylene-C to nourish the cells. A mesh-supported submicron parylene-C membrane (MSPM) structure is design to enhance the mechanical strength of the substrate. In vitro cells culture on the MSPM (with 0.30 μm ultrathin parylene-C) shows that H9-RPE cells are able to adhere, proliferate, form epithelial monolayer with tight intracellular junctions, and become well-polarized with microvilli, which exhibit similar characteristics to RPE cells in vivo. These studies have demonstrated the potential of the MSPM as an artificial Bruch’s membrane for RPE cell transplantation.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1007/s10544-012-9645-8DOIUNSPECIFIED
http://www.springerlink.com/content/p681145387840071/PublisherUNSPECIFIED
ORCID:
AuthorORCID
Humayun, Mark S.0000-0002-5830-5208
Tai, Yu-Chong0000-0001-8529-106X
Additional Information:© 2012 Springer Science+Business Media, LLC. Published online: 4 March 2012. This work is supported by the California Institute of Regenerative Medicine (CIRM); Disease Team Award; Grant DR1-01444.
Funders:
Funding AgencyGrant Number
California Institute for Regenerative Medicine (CIRM)DR1-01444
Subject Keywords:Retina; Parylene-C; Permeability; Cell viability
Issue or Number:4
Record Number:CaltechAUTHORS:20120730-114002078
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20120730-114002078
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:32789
Collection:CaltechAUTHORS
Deposited By: Jason Perez
Deposited On:30 Jul 2012 20:56
Last Modified:09 Mar 2020 13:18

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