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CO_2-inhibition of the amplitude of bending of triton-demembranated sea urchin sperm flagella

Brokaw, C. J. (1977) CO_2-inhibition of the amplitude of bending of triton-demembranated sea urchin sperm flagella. Journal of Experimental Biology, 71 (1). pp. 229-240. ISSN 0022-0949.

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Demembranated sea urchin spermatozoa were reactivated in solutions containing KHCO_3 and observed in a covered well slide. Although KHCO_3 itself causes a small inhibition of flagellar beat frequency, the results confirm previous observations of a direct inhibition of flagellar bend angle by CO_2 with no effect of CO_2 on frequency. Observation of the effect of pH on the inhibition of bend angle in solutions containing KHCO_3 indicates that a given concentration of OH^- has a similar effect to the same concentration of HCO^(-)_3, as would be expected if CO_(2-) inhibition results from reaction of CO_2 with protein-NH^(+)_(3) groups to form carbamates. CO2 may interfere with a control mechanism which selectively suppresses dynein cross-bridge activity in order to generate rhythmic bending. This control mechanism may incorporate a feedback control involving a measure of flagellar amplitude, which fails to operate successfully when the amplitude is reduced below a critical level.

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Additional Information:© 1977 Company of Biologists Ltd. Received 5 May 1977. I thank T. F. Simonick for able assistance with these experiments, and the National Institutes of Health, U.S.P.H.S., for support by grant GM 18711.
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Public Health Service (PHS)GM 18711
Issue or Number:1
Record Number:CaltechAUTHORS:20120801-130446362
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Official Citation:CO2-inhibition of the amplitude of bending of triton-demembranated sea urcin sperm flagella J Exp Biol 1977 71:229-240.
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:32841
Deposited On:01 Aug 2012 22:32
Last Modified:03 Oct 2019 04:04

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