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Nuclear mutations affecting mitochondrial structure and function in Chlamydomonas

Wiseman, Andrew and Gillham, N. W. and Boynton, J. E. (1977) Nuclear mutations affecting mitochondrial structure and function in Chlamydomonas. Journal of Cell Biology, 73 (1). pp. 56-77. ISSN 0021-9525. PMCID PMC2109904. doi:10.1083/jcb.73.1.56.

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Wild type cells of the green alga Chlamydomonas reinhardtii can grow in the in the dark by taking up and respiring exogenously supplied acetate. Obligate photoautotrophic (dark dier, dk) mutants of this alga have been selected which grow at near wild type rates in the light, but rapidly die when transferred to darkness because of defects in mitochondrial structure and function. In crosses of the dk mutants to wild type, the majority of the mutants are inherited in a mendelian fashion, although two have been isolated which are inherited in a clearly nonmendelian fashion. Nine mendelian dk mutants have been analyzed in detail, and belong to eight different complementation groups representing eight gene loci. These mutants have been tentatively grouped into three classes on the basis of the pleiotropic nature of their phenotypic defects. Mutants in Class I have gross alterations in the ultrastructure of their mitochondrial inner membranes together with deficiencies in cytochrome oxidase and antimycin/rotenone-sensitive NADH-cytochrome c reductase activities. Mutants in Class II have a variety of less severe alterations in mitochondrial ultrastructure and deficiencies in cytochrome oxidase activity. Mutants in Class III have normal or near normal mitochondrial ultrastructure and reduced cytochrome oxidase activity. Eight of the nine mutants show corresponding reductions in cyanide-sensitive respiration.

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Additional Information:© 1977 Rockefeller University Press. Received for publication 16 April 1976, and in revised form 22 November 1976. We thank Ms. Sue Fox and Dr. Elizabeth H. Harris for their considerable help in preparing the manuscript for publication. This work was supported by a traineeship to A. Wiseman from Public Health Service Training Grant GM02007, by National Institutes of Health (NIH) Grant GM19427 to J. E. Boynton and N. W. Gillham, and by NIH Research Career Development Awards GM70453 to J. E. Boynton and GM70437 to N. W. Gillham.
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NIH Predoctoral FellowshipGM02007
Issue or Number:1
PubMed Central ID:PMC2109904
Record Number:CaltechAUTHORS:20120803-073534428
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Official Citation:Nuclear mutations affecting mitochondrial structure and function in Chlamydomonas. A Wiseman, N W Gillham, and J E Boynton J Cell Biol 1977 73:56-77. Published April 1, 1977, doi:10.1083/jcb.73.1.56
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:32903
Deposited By: Ruth Sustaita
Deposited On:03 Aug 2012 15:50
Last Modified:09 Nov 2021 21:30

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