CaltechAUTHORS
  A Caltech Library Service

Expression of the Mitochondrial Genome in HeLa Cells XVII. Heterogeneity of Isolated HeLa Cell Mitochondria As Assayed For Their Enzymatic and In Vivo Biosynthetic Activities

Storrie, Brian and Attardi, Giuseppe (1973) Expression of the Mitochondrial Genome in HeLa Cells XVII. Heterogeneity of Isolated HeLa Cell Mitochondria As Assayed For Their Enzymatic and In Vivo Biosynthetic Activities. Journal of Biological Chemistry, 248 (16). pp. 5826-5834. ISSN 0021-9258. https://resolver.caltech.edu/CaltechAUTHORS:20120807-162407308

Full text is not posted in this repository. Consult Related URLs below.

Use this Persistent URL to link to this item: https://resolver.caltech.edu/CaltechAUTHORS:20120807-162407308

Abstract

The distributions in dextran-sucrose or sucrose gradients of biosynthetic and enzymatic activities associated with HeLa cell mitochondria have been analyzed. Mitochondria assayed for their in vivo DNA, RNA, or protein synthetic activity exhibit in such gradients closely corresponding sedimentation profiles, which are all displaced to the heavy side with respect to that of the bulk of mitochondria tested by cytochrome c oxidase activity. The sedimentation profile of mitochondria assayed by DNA content follows fairly precisely that of mitochondria assayed by macromolecular synthetic activities. The distribution in a dextran-sucrose gradient of mitochondria assayed by malate dehydrogenase activity is intermediate between those of mitochondria identified by cytochrome oxidase or in vivo macromolecular biosynthetic activities. An electron microscope examination of the structures fractionated in a dextran-sucrose gradient has given results, which, when correlated with the biochemical data, indicate that HeLa cell mitochondria are active in macromolecular syntheses in proportion to their size, while cytochrome oxidase activity is concentrated in smaller mitochondria. The possibility that an in vivo heterogeneity of HeLa cell mitochondria in DNA content and enzyme activities is responsible for the observed differences in mitochondrial distributions in the dextran-sucrose or sucrose gradients is discussed.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://www.jbc.org/content/248/16/5826PublisherUNSPECIFIED
Additional Information:© 1973 American Society of Biological Chemists, Inc. Received October 12, 1972. The valued assistance of Mrs. B. Keeley and Mrs. LaVerne Wenzel is gratefully acknowledged. This work was supported by Grant GM-11726 from the United States Public Health Service and by a National Science Foundation predoctoral fellowship to B. S. Paper XVI in this series is LEDERMAX, M. & ATTARDI, G. (1973) J. Mol. Biol., in press.
Funders:
Funding AgencyGrant Number
Public Health Service (PHS)GM 11726
NSFUNSPECIFIED
Issue or Number:16
Record Number:CaltechAUTHORS:20120807-162407308
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20120807-162407308
Official Citation:Brian Storrie and Giuseppe Attardi Expression of the Mitochondrial Genome in HeLa Cells: XVII. HETEROGENEITY OF ISOLATED HELA CELL MITOCHONDRIA AS ASSAYED FOR THEIR ENZYMATIC AND IN VIVO BIOSYNTHETIC ACTIVITIES J. Biol. Chem. 1973 248: 5826-5834.
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:32997
Collection:CaltechAUTHORS
Deposited By:INVALID USER
Deposited On:07 Aug 2012 23:42
Last Modified:03 Oct 2019 04:05

Repository Staff Only: item control page