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Live-Cell Imaging of Single Receptor Composition Using Zero-Mode Waveguide Nanostructures

Richards, Christopher I. and Luong, Khai and Srinivasan, Rahul and Turner, Stephen W. and Dougherty, Dennis A. and Korlach, Jonas and Lester, Henry A. (2012) Live-Cell Imaging of Single Receptor Composition Using Zero-Mode Waveguide Nanostructures. Nano Letters, 12 (7). pp. 3690-3694. ISSN 1530-6984. PMCID PMC3397148. doi:10.1021/nl301480h.

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We exploit the optical and spatial features of subwavelength nanostructures to examine individual receptors on the plasma membrane of living cells. Receptors were sequestered in portions of the membrane projected into zero-mode waveguides. Using single-step photobleaching of green fluorescent protein incorporated into individual subunits, the resulting spatial isolation was used to measure subunit stoichiometry in α4β4 and α4β2 nicotinic acetylcholine and P2X2 ATP receptors. We also show that nicotine and cytisine have differential effects on α4β2 stoichiometry.

Item Type:Article
Related URLs:
URLURL TypeDescription CentralArticle
Dougherty, Dennis A.0000-0003-1464-2461
Lester, Henry A.0000-0002-5470-5255
Additional Information:© 2012 American Chemical Society. Received: April 20, 2012. Revised: June 2, 2012. Publication Date (Web): June 5, 2012. We thank A. Fernandez and M. Boitano for assistance in preparing ZMW arrays. Supported by a Beckman Fellowship and an NIH NRSA to C.I.R., by a California Tobacco-Related Disease Research Fellowship to R.S., and by the US National Institutes of Health (NS34407).
Funding AgencyGrant Number
Arnold and Mabel Beckman FoundationUNSPECIFIED
NIH Predoctoral FellowshipUNSPECIFIED
California Tobacco-Related Disease Research ProgramUNSPECIFIED
Subject Keywords:Membrane receptors; nanostructures; single-molecule imaging; nicotinic receptors; zero-mode waveguide
Issue or Number:7
PubMed Central ID:PMC3397148
Record Number:CaltechAUTHORS:20120813-111734004
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Official Citation:Live-Cell Imaging of Single Receptor Composition Using Zero-Mode Waveguide Nanostructures Christopher I. Richards, Khai Luong, Rahul Srinivasan, Stephen W. Turner, Dennis A. Dougherty, Jonas Korlach, and Henry A. Lester Nano Letters 2012 12 (7), 3690-3694
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:33128
Deposited By: Ruth Sustaita
Deposited On:13 Aug 2012 18:36
Last Modified:09 Nov 2021 21:32

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