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Determination of Intracellular pH by ^(31)P Magnetic Resonance

Moon, Richard B. and Richards, John H. (1973) Determination of Intracellular pH by ^(31)P Magnetic Resonance. Journal of Biological Chemistry, 248 (20). pp. 7276-7278. ISSN 0021-9258.

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Observation of the ^(31)P signal from various intracellular phosphates can provide a convenient, nondestructive technique for determining intracellular conditions such as pH. This procedure has been explored with particular reference to the erythrocyte. Both the chemical shift of intracellular inorganic phosphate relative to that of serum phosphate and the positions of, and more especially the difference between, the chemical shifts of 2,3-diphosphoglycerate have been used to monitor intracellular pH of erythrocytes whose hemoglobin has been liganded with carbon monoxide.

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Additional Information:© 1973 American Society of Biological Chemists, Inc. Received for publication, July 5, 1973. This work was supported by Grants NIHL 15198 and NHIL 15162 from the United States Public Health Service. This is Contribution No. 4725 from the Church Laboratory of Chemical Biology.
Funding AgencyGrant Number
Public Health ServiceNIHL 15198
Public Health ServiceNIHL 15162
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Other Numbering System NameOther Numbering System ID
Caltech Church Laboratory of Chemical Biology4725
Issue or Number:20
Record Number:CaltechAUTHORS:20120814-130208418
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Official Citation:Determination of Intracellular pH by 31P Magnetic Resonance Richard B. Moon and John H. Richards J. Biol. Chem. 1973 248: 7276-7278.
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:33175
Deposited By: Ruth Sustaita
Deposited On:14 Aug 2012 20:24
Last Modified:03 Oct 2019 04:07

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