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Stimulation of φX174 production in mitomycin C-treated Escherichia coli cells by caffeine

Steiger, Helmut and Sinsheimer, Robert L. (1968) Stimulation of φX174 production in mitomycin C-treated Escherichia coli cells by caffeine. Journal of Virology, 2 (6). p. 655. ISSN 0022-538X. PMCID PMC375664.

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After pretreatment of an Escherichia coli C culture with mitomycin C, the deoxyribonucleic acid (DNA) synthesis of the bacteria, as well as that of infecting phage φX174, is very largely inhibited. Although the bacterial DNA synthesis is similarly inhibited by mitomycin C in uvr^(-)mutants (P. Howard-Flanders, R. P. Boyce, and L. Theriot, Genetics 53:1119, 1966), phage DNA synthesis and progeny formation take place in a normal manner (B. H. Lindqvist and R. L. Sinsheimer, J. Mol. Biol. 30:69, 1967). The reason for this behavior of the uvr^- cells is not understood. Since it is known that uvr^+ cells do not reactivate double-stranded ultraviolet-damaged phage DNA in the presence of adequate concentrations of caffeine (W. Sauerbier, Biochem. Biophys. Res. Commun. 14:340, 1964), it was of interest to test the ability of mitomycin C-treated uvr^+ cells to produce phage under these conditions.

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Additional Information:© 1968 American Society for Microbiology. Received for publication 28 February 1968. One of us (H.S.) was supported by a NATO fellowship. This research was supported by Public Health Service grant GM13554 from the National Institute of General Medical Sciences.
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North Atlantic Treaty Organization (NATO)UNSPECIFIED
Issue or Number:6
PubMed Central ID:PMC375664
Record Number:CaltechAUTHORS:20120815-133324610
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:33223
Deposited On:15 Aug 2012 21:45
Last Modified:03 Oct 2019 04:08

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