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Use of Radioactive Antibodies for Characterizing Antigens and Application to the Study of Flagella Synthesis

Grant, Geoffrey F. and Simon, Melvin (1968) Use of Radioactive Antibodies for Characterizing Antigens and Application to the Study of Flagella Synthesis. Journal of Bacteriology, 95 (1). pp. 81-86. ISSN 0021-9193. PMCID PMC251974.

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A simple rapid immunochemical procedure has been developed which provides information about the qualitative and quantitative nature of antigens. It involves the use of purified radioactive (^(125)I-labeled) antibodies. The amount of antibody bound to the antigen is determined by filtering the mixture through diethylaminoethyl (DEAE)-cellulose paper. All of the antigen, as well as the antibody complexed with it, is trapped on the paper, whereas free antibody is removed by repeated washing. This technique has been applied to the study of three immune systems, bovine serum albumin, Escherichia coli tryptophan synthetase B protein, and Bacillus subtilis flagella. The results obtained by the DEAE-antibody binding technique were comparable, in terms of sensitivity, specificity, and accuracy, to data obtained by microcomplement fixation and precipitin methods. The assay was used to measure the kinetics of flagella regeneration in B. subtilis.

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Additional Information:© 1968 American Society for Microbiology. Received for publication 19 October 1967. The authors would like to express their gratitude to K. Dimmitt, for both innumerable valuable suggestions and expert technical assistance, and to L. Huggett, for carrying out the complement-fixation experiments. This investigation was supported by a grant from the National Science Foundation (GB-4153).
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Issue or Number:1
PubMed Central ID:PMC251974
Record Number:CaltechAUTHORS:20120816-093725686
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:33255
Deposited On:16 Aug 2012 17:46
Last Modified:03 Oct 2019 04:08

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