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A Multimode Optical Imaging System for Preclinical Applications In Vivo: Technology Development, Multiscale Imaging, and Chemotherapy Assessment

Hwang, Jae Youn and Wachsmann-Hogiu, Sebastian and Ramanujan, V. Krishnan and Ljubimova, Julia and Gross, Zeev and Gray, Harry B. and Medina-Kauwe, Lali K. and Farkas, Daniel L. (2012) A Multimode Optical Imaging System for Preclinical Applications In Vivo: Technology Development, Multiscale Imaging, and Chemotherapy Assessment. Molecular Imaging and Biology, 14 (4). pp. 431-442. ISSN 1536-1632. PMCID PMC3487699. http://resolver.caltech.edu/CaltechAUTHORS:20120817-134341510

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Abstract

Purpose: Several established optical imaging approaches have been applied, usually in isolation, to preclinical studies; however, truly useful in vivo imaging may require a simultaneous combination of imaging modalities to examine dynamic characteristics of cells and tissues. We developed a new multimode optical imaging system designed to be application-versatile, yielding high sensitivity, and specificity molecular imaging. Procedures: We integrated several optical imaging technologies, including fluorescence intensity, spectral, lifetime, intravital confocal, two-photon excitation, and bioluminescence, into a single system that enables functional multiscale imaging in animal models. Results: The approach offers a comprehensive imaging platform for kinetic, quantitative, and environmental analysis of highly relevant information, with micro-to-macroscopic resolution. Applied to small animals in vivo, this provides superior monitoring of processes of interest, represented here by chemo-/nanoconstruct therapy assessment. Conclusions: This new system is versatile and can be optimized for various applications, of which cancer detection and targeted treatment are emphasized here.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1007/s11307-011-0517-zDOIArticle
http://www.springerlink.com/content/8019061262320485/?MUD=MPPublisherArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3487699PubMed CentralArticle
Additional Information:© 2011 World Molecular Imaging Society. Published Online: 27 August 2011. We thank Dr. Mark Gaon for help developing and testing the gated anesthesia instrument. Some of this work was done in partial fulfillment of Ph.D. thesis research requirements by Dr. J.Y. Hwang, at the University of Southern California. Work at the California Institute of Technology was supported by the Arnold and Mabel Beckman Foundation. Z.G. thanks Johnson & Johnson for research support. We are grateful for the following federal support of our research: NIH (5R01CA123495-03 and 1U01CA151815-0) to JYL; NIH (1R01 CA140995 and 1R01 CA129822) and DOD W81XWH-06-1-0549 to LKMK; and US Navy Bureau of Medicine and Surgery (1435-04-04-GT-41387 and -43096), NIH (N01-CO- 07119), and NSF (BESOO 79483) to DLF. Conflict of Interest. The authors declare they have no conflicts of interest.
Funders:
Funding AgencyGrant Number
Arnold and Mabel Beckman FoundationUNSPECIFIED
Johnson & JohnsonUNSPECIFIED
NIH5R01CA123495-03
NIH1U01CA151815-0
NIH1R01 CA140995
NIH1R01 CA129822
Department of DefenseW81XWH-06-1-0549
U. S. Navy Bureau of Medicine and Surgery1435-04-04-GT-41387
U. S. Navy Bureau of Medicine and Surgery1435-04-04-GT-43096
NIHN01-CO- 07119
NSFBESOO 79483
Subject Keywords:Multimode; Preclinical; In vivo; Spectral analysis; Fluorescence lifetime; Wide-field two-photon excitation; Chemotherapy; Nanoconstruct; Corroles
PubMed Central ID:PMC3487699
Record Number:CaltechAUTHORS:20120817-134341510
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20120817-134341510
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:33303
Collection:CaltechAUTHORS
Deposited By: Jason Perez
Deposited On:17 Aug 2012 22:10
Last Modified:05 Jun 2017 21:32

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