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Spectral-domain phase microscopy

Choma, Michael A. and Ellerbee, Audrey K. and Yang, Changhuei and Creazzo, Tony L. and Izatt, Joseph A. (2005) Spectral-domain phase microscopy. Optics Letters, 30 (10). pp. 1162-1164. ISSN 0146-9592.

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Broadband interferometry is an attractive technique for the detection of cellular motions because it provides depth-resolved phase information via coherence gating. We present a phase-sensitive technique called spectral-domain phase microscopy (SDPM). SDPM is a functional extension of spectral-domain optical coherence tomography that allows for the detection of nanometer-scale motions in living cells. The sensitivity of the technique is demonstrated, and its calibration is verified. A shot-noise limit to the displacement sensitivity of this technique is derived. Measurement of cellular dynamics was performed on spontaneously beating cardiomyocytes isolated from chick embryos.

Item Type:Article
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Yang, Changhuei0000-0001-8791-0354
Additional Information:© 2005 Optical Society of America Received October 8, 2004 This work was supported by National Institutes of Health grants R24-EB000243 and R21-RR019769 to J. A. Izatt and grants R01 HL715015 and P01 HL36059 to T. L. Creazzo.
Funding AgencyGrant Number
NIHR01 HL715015
NIHP01 HL36059
Subject Keywords:Noise in imaging systems; Optical coherence tomography; Interferometry; Interference microscopy
Issue or Number:10
Record Number:CaltechAUTHORS:CHOol05c
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:3332
Deposited By: Archive Administrator
Deposited On:31 May 2006
Last Modified:09 Mar 2020 13:19

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