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Metalloprotein-based contrast sensors for neurochemical detection by MRI

Lelyveld, Victor and Brustad, Eric and Arnold, Frances and Jasanoff, Alan (2012) Metalloprotein-based contrast sensors for neurochemical detection by MRI. Protein Science, 21 (S1). p. 184. ISSN 0961-8368.

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Noninvasive imaging methods to observe brain activity at a molecular level would be of significant benefit to neuroscience and the study of neurological disorders. To this end, we report several advances toward sensing biomolecules important in neurotransmission, neuromodulation, and inflammation by proton magnetic resonance imaging (1-H MRI). These approaches rely on exploiting the biomolecular specificity of naturally and artificially evolved protein binding pockets, as well as the natural and augmented proton spin relaxation effects of paramagnetic metalloproteins. Cytochrome P450 hemedomain mutants have been screened to reveal a set of turn-off MRI sensors for monoamine neurotransmitters with micromolar to submicromolar affinity, for which specific Type II ligand binding confers as much as a 93% change in T1 contrast enhancement at 4.7 T.

Item Type:Article
Brustad, Eric0000-0002-7031-5433
Arnold, Frances0000-0002-4027-364X
Jasanoff, Alan0000-0002-2834-6359
Issue or Number:S1
Record Number:CaltechAUTHORS:20120827-083652928
Persistent URL:
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:33545
Deposited By: Tony Diaz
Deposited On:27 Aug 2012 22:22
Last Modified:09 Mar 2020 13:18

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