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Single-protein nanomechanical mass spectrometry in real time

Hanay, M. S. and Kelber, S. and Naik, A. K. and Chi, D. and Hentz, S. and Bullard, E. C. and Colinet, E. and Duraffourg, L. and Roukes, M. L. (2012) Single-protein nanomechanical mass spectrometry in real time. Nature Nanotechnology, 7 (9). pp. 602-608. ISSN 1748-3387. PMCID PMC3435450. http://resolver.caltech.edu/CaltechAUTHORS:20120827-100927277

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Abstract

Nanoelectromechanical systems (NEMS) resonators can detect mass with exceptional sensitivity. Previously, mass spectra from several hundred adsorption events were assembled in NEMS-based mass spectrometry using statistical analysis. Here, we report the first realization of single-molecule NEMS-based mass spectrometry in real time. As each molecule in the sample adsorbs on the resonator, its mass and position of adsorption are determined by continuously tracking two driven vibrational modes of the device. We demonstrate the potential of multimode NEMS-based mass spectrometry by analysing IgM antibody complexes in real time. NEMS-based mass spectrometry is a unique and promising new form of mass spectrometry: it can resolve neutral species, provide a resolving power that increases markedly for very large masses, and allow the acquisition of spectra, molecule-by-molecule, in real time.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1038/nnano.2012.119DOIArticle
http://www.nature.com/nnano/journal/vaop/ncurrent/full/nnano.2012.119.htmlPublisherArticle
http://rdcu.be/cseNPublisherFree ReadCube access
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3435450/PubMed CentralArticle
ORCID:
AuthorORCID
Roukes, M. L.0000-0002-2916-6026
Additional Information:© 2012 Macmillan Publishers Limited. Received 24 February 2012; Accepted 15 June 2012; Published online 26 August 2012. The authors thank I. Bargatin, E. Myers, M. Shahgholi, I. Kozinsky, M. Matheny, J. Sader, P. Hung, E. Sage and R. Karabalin for helpful discussions, and C. Marcoux for assistance with device fabrication. The authors acknowledge the support and infrastructure provided by the Kavli Nanoscience Institute at Caltech, as well as support from the NIH (grant no. R01-GM085666-01A1Z), the NSF (MRI grant no. DBI-0821863), the Fondation pour la Recherche et l’Enseignement Superieur, an Institut Mérieux Research Grant, partial support from the Institut Carnot CEA-LETI and the Carnot-NEMS project, and a grant from the Partnership University Fund of the French Embassy to the USA. M.L.R. acknowledges support from an NIH Director’s Pioneer Award and a Chaire d’Excellence (RTRA) from Fondation Nanosciences. S.H. and E.C. acknowledge partial support from EU CEA Eurotalent Fellowships. Author contributions: M.L.R., A.K.N., M.S.H. and S.K. conceived and designed the experiments. M.S.H., S.K. and A.K.N. performed the experiments. M.S.H., S.K., A.K.N. and M.L.R. analysed the data. M.S.H., S.K., A.K.N., D.C., S.H., E.C.B., E.C., L.D. and M.L.R. contributed materials and analysis tools. M.S.H., S.K., M.L.R. and A.K.N. wrote the paper.
Group:Kavli Nanoscience Institute
Funders:
Funding AgencyGrant Number
NIHR01-GM085666-01A1Z
NSFDBI-0821863
Fondation pour la Recherche et l’Enseignement SuperieurUNSPECIFIED
Institut Mérieux Research GrantUNSPECIFIED
Institut Carnot CEA-LETIUNSPECIFIED
Carnot-NEMS ProjectUNSPECIFIED
French Embassy Partnership University FundUNSPECIFIED
NIH Director’s Pioneer AwardUNSPECIFIED
Fondation Nanosciences Chaire d’Excellence (RTRA)UNSPECIFIED
EU CEA Eurotalent FellowshipsUNSPECIFIED
Kavli Nanoscience InstituteUNSPECIFIED
PubMed Central ID:PMC3435450
Record Number:CaltechAUTHORS:20120827-100927277
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20120827-100927277
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:33554
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:27 Aug 2012 17:59
Last Modified:03 Nov 2017 20:45

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