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Single-protein nanomechanical mass spectrometry in real time

Hanay, M. S. and Kelber, S. and Naik, A. K. and Chi, D. and Hentz, S. and Bullard, E. C. and Colinet, E. and Duraffourg, L. and Roukes, M. L. (2012) Single-protein nanomechanical mass spectrometry in real time. Nature Nanotechnology, 7 (9). pp. 602-608. ISSN 1748-3387. PMCID PMC3435450. doi:10.1038/nnano.2012.119.

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Nanoelectromechanical systems (NEMS) resonators can detect mass with exceptional sensitivity. Previously, mass spectra from several hundred adsorption events were assembled in NEMS-based mass spectrometry using statistical analysis. Here, we report the first realization of single-molecule NEMS-based mass spectrometry in real time. As each molecule in the sample adsorbs on the resonator, its mass and position of adsorption are determined by continuously tracking two driven vibrational modes of the device. We demonstrate the potential of multimode NEMS-based mass spectrometry by analysing IgM antibody complexes in real time. NEMS-based mass spectrometry is a unique and promising new form of mass spectrometry: it can resolve neutral species, provide a resolving power that increases markedly for very large masses, and allow the acquisition of spectra, molecule-by-molecule, in real time.

Item Type:Article
Related URLs:
URLURL TypeDescription ReadCube access CentralArticle
Hanay, M. S.0000-0002-1928-044X
Hentz, S.0000-0003-0479-9027
Duraffourg, L.0000-0002-9829-9295
Roukes, M. L.0000-0002-2916-6026
Additional Information:© 2012 Macmillan Publishers Limited. Received 24 February 2012; Accepted 15 June 2012; Published online 26 August 2012. The authors thank I. Bargatin, E. Myers, M. Shahgholi, I. Kozinsky, M. Matheny, J. Sader, P. Hung, E. Sage and R. Karabalin for helpful discussions, and C. Marcoux for assistance with device fabrication. The authors acknowledge the support and infrastructure provided by the Kavli Nanoscience Institute at Caltech, as well as support from the NIH (grant no. R01-GM085666-01A1Z), the NSF (MRI grant no. DBI-0821863), the Fondation pour la Recherche et l’Enseignement Superieur, an Institut Mérieux Research Grant, partial support from the Institut Carnot CEA-LETI and the Carnot-NEMS project, and a grant from the Partnership University Fund of the French Embassy to the USA. M.L.R. acknowledges support from an NIH Director’s Pioneer Award and a Chaire d’Excellence (RTRA) from Fondation Nanosciences. S.H. and E.C. acknowledge partial support from EU CEA Eurotalent Fellowships. Author contributions: M.L.R., A.K.N., M.S.H. and S.K. conceived and designed the experiments. M.S.H., S.K. and A.K.N. performed the experiments. M.S.H., S.K., A.K.N. and M.L.R. analysed the data. M.S.H., S.K., A.K.N., D.C., S.H., E.C.B., E.C., L.D. and M.L.R. contributed materials and analysis tools. M.S.H., S.K., M.L.R. and A.K.N. wrote the paper.
Group:Kavli Nanoscience Institute
Funding AgencyGrant Number
Fondation pour la Recherche et l’Enseignement SuperieurUNSPECIFIED
Institut Mérieux Research GrantUNSPECIFIED
French Embassy Partnership University FundUNSPECIFIED
NIH Director’s Pioneer AwardUNSPECIFIED
Fondation Nanosciences Chaire d’Excellence (RTRA)UNSPECIFIED
EU CEA Eurotalent FellowshipsUNSPECIFIED
Kavli Nanoscience InstituteUNSPECIFIED
Issue or Number:9
PubMed Central ID:PMC3435450
Record Number:CaltechAUTHORS:20120827-100927277
Persistent URL:
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:33554
Deposited By: Tony Diaz
Deposited On:27 Aug 2012 17:59
Last Modified:09 Nov 2021 21:35

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