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Characterization and role of carbonic anhydrase in the calcification process of the azooxanthellate coral Tubastrea aurea

Tambutté, Sylvie and Tambutté, Eric and Zoccola, Didier and Caminiti, Natacha and Lotto, Severine and Moya, Aurélie and Allemand, Denis and Adkins, Jess (2007) Characterization and role of carbonic anhydrase in the calcification process of the azooxanthellate coral Tubastrea aurea. Marine Biology, 151 (1). pp. 71-83. ISSN 0025-3162. https://resolver.caltech.edu/CaltechAUTHORS:20120828-153725990

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Abstract

In zooxanthellate corals, the photosynthetic fixation of carbon dioxide and the precipitation of CaCO_3 are intimately linked both spatially and temporally making it difficult to study carbon transport mechanisms involved in each pathway. When studying Tubastrea aurea, a coral devoid of zooxanthellae, we can focus on carbon transport mechanisms involved only in the calcification process. We performed this study to characterize T. aurea carbonic anhydrase and to determine its role in the calcification process. We have shown that inhibition of tissular carbonic anhydrase activity affects the calcification rate. We have measured the activity of this enzyme both in the tissues and in the organix matrix extracted from the skeleton. Our results indicate that organic matrix proteins, which are synthesized by the calcifying tissues, are not only structural proteins, but they also play a crucial catalytic role by eliminating the kinetic barrier to interconversion of inorganic carbon at the calcification site. By immunochemistry we have demonstrated the presence of a protein both in the tissues and in the organic matrix, which shares common features with prokaryotic carbonic anhydrases.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1007/s00227-006-0452-8DOIUNSPECIFIED
http://www.springerlink.com/content/g8356886046h222n/PublisherUNSPECIFIED
ORCID:
AuthorORCID
Adkins, Jess0000-0002-3174-5190
Additional Information:© 2006 Springer-Verlag. Received: 8 May 2006; Accepted: 9 August 2006; Published online: 30 September 2006. We thank Prof. François Morel from Princeton University and Mak Saïto from the Woods Hole Oceanographic Institution for providing the antibody, anti-bcarbonic anhydrase from Synecchococcus sp. This study was conducted as part of the Centre Scientifique de Monaco 2000–2004 research program. It was supported by the Government of the Principality of Monaco and by the California Institute of Technology, USA.
Funders:
Funding AgencyGrant Number
Government of the Principality of MonacoUNSPECIFIED
CaltechUNSPECIFIED
Subject Keywords:Carbonic anhydrase; Carbon; Calcification; Coral; Biomineralization; Organic matrix
Issue or Number:1
Record Number:CaltechAUTHORS:20120828-153725990
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20120828-153725990
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:33629
Collection:CaltechAUTHORS
Deposited By: Jason Perez
Deposited On:29 Aug 2012 15:06
Last Modified:24 Feb 2020 10:30

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