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A Universal HPLC-MS Method to Determine the Stereochemistry of Common and Unusual Amino Acids

Hess, Sonja (2012) A Universal HPLC-MS Method to Determine the Stereochemistry of Common and Unusual Amino Acids. In: Amino Acid Analysis: Methods and Protocols. Methods in Molecular Biology. No.828. Springer , London, pp. 63-75. ISBN 9781617794452.

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The determination of the stereochemistry of common and unusual amino acids is important in food chemistry, archeology, medicine, and life sciences, including such diverse areas as marine biology and extraterrestrial chemistry and has greatly contributed to our current knowledge in these fields. To determine the stereochemistry of amino acids, many chromatographic methods have been developed and refined over the last decades. Here, we describe a state-of-the-art indirect chromatography-based LC-MS method. Diastereomers were formed from amino acids that were reacted with chiral derivatizing agents, such as Marfey’s reagent (FDAA), GITC, S-NIFE, and OPA-IBLC and separated on a reversed phase column using mass spectrometry compatible buffers.

Item Type:Book Section
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URLURL TypeDescription
Hess, Sonja0000-0002-5904-9816
Additional Information:© 2012 Springer Science+Business Media, LLC. This work was supported by the Intramural Program of the National Institute of Diabetes, Digestive and Kidney Diseases, Z01 DK070004-04, the Beckman Institute and the Betty and Gordon Moore Foundation.
Funding AgencyGrant Number
National Institute of Diabetes, Digestive and Kidney DiseasesZ01 DK070004-04
Caltech Beckman InstituteUNSPECIFIED
Gordon and Betty Moore FoundationUNSPECIFIED
Subject Keywords:Amino acid derivatization, Enantiomer, Marfey’s reagent, FDAA, GITC, S-NIFE, OPA-IBLC, Amino acid analysis
Series Name:Methods in Molecular Biology
Issue or Number:828
Record Number:CaltechAUTHORS:20120917-112748155
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:34143
Deposited By: Tony Diaz
Deposited On:17 Sep 2012 19:04
Last Modified:03 Oct 2019 04:16

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