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A long-term in vitro silicon-based microelectrode-neuron connection

Regehr, Wade G. and Pine, Jerome and Rutledge, David B. (1988) A long-term in vitro silicon-based microelectrode-neuron connection. IEEE Transactions on Biomedical Engineering, 35 (12). pp. 1023-1032. ISSN 0018-9294.

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A novel method for long-term recording and simulation applicable to cultured neurons has been developed. Silicon-based microelectrodes have been fabricated using integrated-circuit technology and micromachining. The chronic connection is made by positioning the tip of the `diving-board electrode' into contact with the top of the cell body. The electrode support structure is then glued to the bottom of the culture dish. Two-way electrical connections to Helisoma B19 neurons have been maintained for up to four days. This capability makes it possible to conduct experiments that are not practical using conventional techniques.

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Additional Information:© Copyright 2006 IEEE. Reprinted with permission. Manuscript received February 29, 1988; revised June 22, 1988. This work was supported by NSF Grant BNS8603713. The authors are grateful to Dr. S . B. Kater for supplying Helisoma neurons and for making his laboratory available for experiments, and to Dr. C. Cohan for helping with the Helisoma physiology.
Subject Keywords:2-way electrical connections; Helisoma B19 neurons; Si-based microelectrode neuron connection; diving-board electrode; electrode support structure; integrated-circuit technology; micromachining; neuroscience method
Issue or Number:12
Record Number:CaltechAUTHORS:REGieeetbe88
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:3432
Deposited By: Archive Administrator
Deposited On:06 Jun 2006
Last Modified:02 Oct 2019 23:03

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