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Time-lapse microscopy of macrophages during embryonic vascular development

Al-Roubaie, Sarah and Hughes, Jasmine H. and Filla, Michael B. and Lansford, Rusty and Lehoux, Stephanie and Jones, Elizabeth A. V. (2012) Time-lapse microscopy of macrophages during embryonic vascular development. Developmental Dynamics, 241 (9). pp. 1423-1431. ISSN 1058-8388.

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Background: Macrophages are present before the onset of blood flow, but very little is known about their function in vascular development. We have developed a technique to concurrently label both endothelial cells and macrophages for time-lapse microscopy using co-injection of fluorescently conjugated acetylated low-density lipoprotein (AcLDL) and phagocytic dye PKH26-PCL. Results: We characterize double-labeled cells to confirm specific labeling of macrophages. Double-labeled cells circulate, roll along the endothelium, and extravasate from vessels. Most observed macrophages are integrated into the vessel wall, showing an endothelial-like morphology. We used transgenic quail that express a fluorescent protein driven by the endothelial-specific promoter Tie1 in conjugation with the phagocytic dye to analyze these cells. Circulating PKH26-PCL-labeled cells are mostly Tie1−, but those which have integrated into the vessel wall are largely Tie1+. The endothelial-like phagocytic cells were generally stationary during normal vascular development. We, therefore, induced vascular remodeling and found that these cells could be recruited to sites of remodeling. Conclusions: The active interaction of endothelial cells and macrophages support the hypothesis that these cells are involved in vascular remodeling. The presence of phagocytic endothelial-like cells suggests either a myeloid-origin to certain endothelial cells or that circulating endothelial cells/hematopoietic stem cells have phagocytic capacity.

Item Type:Article
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URLURL TypeDescription DOIArticle
Lansford, Rusty0000-0002-2159-3699
Additional Information:© 2012 Wiley Periodicals, Inc. Accepted 5 July 2012. Article first published online: 30 Jul. 2012. Grant sponsor: NSERC Discovery Program; Grant number: 342134; Grant sponsor: Canadian Foundation for Innovation; Grant sponsor: the Canada Research Chair Program. S.A.R. was supported by the Eugenie Lamothe Fellowship.
Funding AgencyGrant Number
Eugenie Lamothe FellowshipUNSPECIFIED
Natural Sciences and Engineering Research Council of Canada (NSERC)342134
Canada Foundation for InnovationUNSPECIFIED
Canada Research Chair ProgramUNSPECIFIED
Subject Keywords:vascular development; in vivo imaging; fluorescent cell-labeling; endothelial cells; macrophages; phagocytes
Issue or Number:9
Record Number:CaltechAUTHORS:20120927-130109788
Persistent URL:
Official Citation:Al-Roubaie, S., Hughes, J. H., Filla, M. B., Lansford, R., Lehoux, S. and Jones, E. A.V. (2012), Time-lapse microscopy of macrophages during embryonic vascular development. Dev. Dyn., 241: 1423–1431. doi: 10.1002/dvdy.23835
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:34514
Deposited By: Tony Diaz
Deposited On:27 Sep 2012 20:14
Last Modified:03 Oct 2019 04:19

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