CaltechAUTHORS
  A Caltech Library Service

Time-lapse microscopy of macrophages during embryonic vascular development

Al-Roubaie, Sarah and Hughes, Jasmine H. and Filla, Michael B. and Lansford, Rusty and Lehoux, Stephanie and Jones, Elizabeth A. V. (2012) Time-lapse microscopy of macrophages during embryonic vascular development. Developmental Dynamics, 241 (9). pp. 1423-1431. ISSN 1058-8388. https://resolver.caltech.edu/CaltechAUTHORS:20120927-130109788

Full text is not posted in this repository. Consult Related URLs below.

Use this Persistent URL to link to this item: https://resolver.caltech.edu/CaltechAUTHORS:20120927-130109788

Abstract

Background: Macrophages are present before the onset of blood flow, but very little is known about their function in vascular development. We have developed a technique to concurrently label both endothelial cells and macrophages for time-lapse microscopy using co-injection of fluorescently conjugated acetylated low-density lipoprotein (AcLDL) and phagocytic dye PKH26-PCL. Results: We characterize double-labeled cells to confirm specific labeling of macrophages. Double-labeled cells circulate, roll along the endothelium, and extravasate from vessels. Most observed macrophages are integrated into the vessel wall, showing an endothelial-like morphology. We used transgenic quail that express a fluorescent protein driven by the endothelial-specific promoter Tie1 in conjugation with the phagocytic dye to analyze these cells. Circulating PKH26-PCL-labeled cells are mostly Tie1−, but those which have integrated into the vessel wall are largely Tie1+. The endothelial-like phagocytic cells were generally stationary during normal vascular development. We, therefore, induced vascular remodeling and found that these cells could be recruited to sites of remodeling. Conclusions: The active interaction of endothelial cells and macrophages support the hypothesis that these cells are involved in vascular remodeling. The presence of phagocytic endothelial-like cells suggests either a myeloid-origin to certain endothelial cells or that circulating endothelial cells/hematopoietic stem cells have phagocytic capacity.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1002/dvdy.23835 DOIArticle
http://onlinelibrary.wiley.com/doi/10.1002/dvdy.23835/abstractPublisherArticle
ORCID:
AuthorORCID
Lansford, Rusty0000-0002-2159-3699
Additional Information:© 2012 Wiley Periodicals, Inc. Accepted 5 July 2012. Article first published online: 30 Jul. 2012. Grant sponsor: NSERC Discovery Program; Grant number: 342134; Grant sponsor: Canadian Foundation for Innovation; Grant sponsor: the Canada Research Chair Program. S.A.R. was supported by the Eugenie Lamothe Fellowship.
Funders:
Funding AgencyGrant Number
Eugenie Lamothe FellowshipUNSPECIFIED
Natural Sciences and Engineering Research Council of Canada (NSERC)342134
Canada Foundation for InnovationUNSPECIFIED
Canada Research Chair ProgramUNSPECIFIED
Subject Keywords:vascular development; in vivo imaging; fluorescent cell-labeling; endothelial cells; macrophages; phagocytes
Issue or Number:9
Record Number:CaltechAUTHORS:20120927-130109788
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20120927-130109788
Official Citation:Al-Roubaie, S., Hughes, J. H., Filla, M. B., Lansford, R., Lehoux, S. and Jones, E. A.V. (2012), Time-lapse microscopy of macrophages during embryonic vascular development. Dev. Dyn., 241: 1423–1431. doi: 10.1002/dvdy.23835
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:34514
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:27 Sep 2012 20:14
Last Modified:03 Oct 2019 04:19

Repository Staff Only: item control page