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Evaluating the thermostability of commercial fast real-time PCR master mixes

Abou Tayoun, Ahmad N. and Ward, Brian P. and Maltezos, George and Scherer, Axel and Tsongalis, Gregory J. (2012) Evaluating the thermostability of commercial fast real-time PCR master mixes. Experimental and Molecular Pathology, 93 (2). pp. 261-263. ISSN 0014-4800. doi:10.1016/j.yexmp.2012.05.002. https://resolver.caltech.edu/CaltechAUTHORS:20120927-134014576

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Abstract

There is an increasing need for affordable, thermostable PCR reagents that can be used for diagnostic testing in resource limited settings. The development of point-of-care devices in such settings is highly dependent on the availability and efficacy of thermostable reagents. Here, we assess the thermostability of commercial, intercalating dye-based real-time PCR master mixes. We show that several of these master mixes have thermostability and robust performance at 20 °C, 40 °C, and 45 °C for 6, 4 and 2 weeks, respectively. However, none of the master mixes that we evaluated was able to withstand more than 1 month at 45 °C incubation. Our findings have implications for clinical diagnostics in the developing world where cold-chain delivery of diagnostic assays is difficult to guarantee.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/j.yexmp.2012.05.002 DOIUNSPECIFIED
http://www.sciencedirect.com/science/article/pii/S0014480012000767PublisherUNSPECIFIED
Additional Information:© 2012 Elsevier Inc. Received 3 May 2012; Available online 10 May 2012. This work was supported by the Bill and Melinda gates Foundation [Global Health Grant Number OPP1028794]. Funding for open access charge: Bill and Melinda Gates Foundation.
Funders:
Funding AgencyGrant Number
Bill and Melinda Gates FoundationOPP1028794
Subject Keywords:Master mix; PCR; Thermostability
Issue or Number:2
DOI:10.1016/j.yexmp.2012.05.002
Record Number:CaltechAUTHORS:20120927-134014576
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20120927-134014576
Official Citation:Ahmad N. Abou Tayoun, Brian P. Ward, George Maltezos, Axel Scherer, Gregory J. Tsongalis, Evaluating the thermostability of commercial fast real-time PCR master mixes, Experimental and Molecular Pathology, Volume 93, Issue 2, October 2012, Pages 261-263, ISSN 0014-4800, 10.1016/j.yexmp.2012.05.002.
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:34521
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:27 Sep 2012 20:46
Last Modified:09 Nov 2021 23:09

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