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Direct and in direct control of oral ectoderm regulatory gene expression by Nodal signaling in the sea urchin embryo

Li, Enhu and Materna, Stefan C. and Davidson, Eric H. (2012) Direct and in direct control of oral ectoderm regulatory gene expression by Nodal signaling in the sea urchin embryo. Developmental Biology, 369 (2). pp. 377-385. ISSN 0012-1606. PMCID PMC3423475. doi:10.1016/j.ydbio.2012.06.022. https://resolver.caltech.edu/CaltechAUTHORS:20121005-085219647

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Abstract

The Nodal signaling pathway is known from earlier work to be an essential mediator of oral ectoderm specification in the sea urchin embryo, and indirectly, of aboral ectoderm specification as well. Following expression of the Nodal ligand in the future oral ectoderm during cleavage, a sequence of regulatory gene activations occur within this territory which depend directly or indirectly on nodal gene expression. Here we describe additional regulatory genes that contribute to the oral ectoderm regulatory state during specification in Strongylocentrotus purpuratus, and show how their spatial expression changes dynamically during development. By means of system wide perturbation analyses we have significantly improved current knowledge of the epistatic relations among the regulatory genes of the oral ectoderm. From these studies there emerge diverse circuitries relating downstream regulatory genes directly and indirectly to Nodal signaling. A key intermediary regulator, the role of which had not previously been discerned, is the not gene. In addition to activating several genes earlier described as targets of Nodal signaling, the not gene product acts to repress other oral ectoderm genes, contributing crucially to the bilateral spatial organization of the embryonic oral ectoderm.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/j.ydbio.2012.06.022DOIArticle
http://www.sciencedirect.com/science/article/pii/S001216061200351XPublisherArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3423475/PubMed CentralArticle
Additional Information:© 2012 Elsevier Inc. Received 25 April 2012. Received in revised form 19 June 2012. Accepted 27 June 2012. Available online 6 July 2012. We thank Dr. Michael Collins for helpful discussions and critiques. This work was supported by NIH Grant HD-037105.
Funders:
Funding AgencyGrant Number
NIHHD-37105
Subject Keywords:Nodal signaling; Gene regulatory network; Oral ectoderm specification
Issue or Number:2
PubMed Central ID:PMC3423475
DOI:10.1016/j.ydbio.2012.06.022
Record Number:CaltechAUTHORS:20121005-085219647
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20121005-085219647
Official Citation:Enhu Li, Stefan C. Materna, Eric H. Davidson, Direct and indirect control of oral ectoderm regulatory gene expression by Nodal signaling in the sea urchin embryo, Developmental Biology, Volume 369, Issue 2, 15 September 2012, Pages 377-385, ISSN 0012-1606, 10.1016/j.ydbio.2012.06.022. (http://www.sciencedirect.com/science/article/pii/S001216061200351X)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:34697
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:05 Oct 2012 18:03
Last Modified:09 Nov 2021 23:10

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