Hodas, Jennifer J. L. and Nehring, Anne and Höche, Nicole and Sweredoski, Michael J. and Pielot, Rainer and Hess, Sonja and Tirrell, David A. and Dieterich, Daniela C. and Schuman, Erin M. (2012) Dopaminergic modulation of the hippocampal neuropil proteome identified by bioorthogonal noncanonical amino acid tagging (BONCAT). Proteomics, 12 (15-16). pp. 2464-2476. ISSN 1615-9853. PMCID PMC3752339. doi:10.1002/pmic.201200112. https://resolver.caltech.edu/CaltechAUTHORS:20121005-102359212
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Abstract
Local protein synthesis and its activity-dependent modulation via dopamine receptor stimulation play an important role in synaptic plasticity – allowing synapses to respond dynamically to changes in their activity patterns. We describe here the metabolic labeling, enrichment, and MS-based identification of candidate proteins specifically translated in intact hippocampal neuropil sections upon treatment with the selective D1/D5 receptor agonist SKF81297. Using the noncanonical amino acid azidohomoalanine and click chemistry, we identified over 300 newly synthesized proteins specific to dendrites and axons. Candidates specific for the SKF81297-treated samples were predominantly involved in protein synthesis and synapse-specific functions. Furthermore, we demonstrate a dendrite-specific increase in proteins synthesis upon application of SKF81297. This study provides the first snapshot in the dynamics of the dopaminergic hippocampal neuropil proteome.
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Additional Information: | © 2012 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim. Received: March 11, 2012. Revised: May 21, 2012. Accepted: May 22, 2012. Article first published online: 20 Aug. 2012. This work was supported by the German Academy for Natural Scientists Leopoldina and the DFG (Emmy Noether Program and a DIP grant to D. C. D.), the NIH (E. M. S. and D. A. T. 5R01GM62523–9), HHMI (E. M. S.), Gordon and Betty Moore Foundation (M. J. S., S. H.), and the Beckman Institute (M. J. S, S. H.). | ||||||||||||||
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Subject Keywords: | Animal proteomics; BONCAT; Click chemistry; Dopaminergic signaling; Local protein synthesis; Metabolic labeling; MUDPIT | ||||||||||||||
Issue or Number: | 15-16 | ||||||||||||||
PubMed Central ID: | PMC3752339 | ||||||||||||||
DOI: | 10.1002/pmic.201200112 | ||||||||||||||
Record Number: | CaltechAUTHORS:20121005-102359212 | ||||||||||||||
Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20121005-102359212 | ||||||||||||||
Official Citation: | Hodas, J. J. L., Nehring, A., Höche, N., Sweredoski, M. J., Pielot, R., Hess, S., Tirrell, D. A., Dieterich, D. C. and Schuman, E. M. (2012), Dopaminergic modulation of the hippocampal neuropil proteome identified by bioorthogonal noncanonical amino acid tagging (BONCAT). Proteomics, 12: 2464–2476. doi: 10.1002/pmic.201200112 | ||||||||||||||
Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | ||||||||||||||
ID Code: | 34705 | ||||||||||||||
Collection: | CaltechAUTHORS | ||||||||||||||
Deposited By: | Tony Diaz | ||||||||||||||
Deposited On: | 10 Oct 2012 18:23 | ||||||||||||||
Last Modified: | 09 Nov 2021 23:10 |
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