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Dopaminergic modulation of the hippocampal neuropil proteome identified by bioorthogonal noncanonical amino acid tagging (BONCAT)

Hodas, Jennifer J. L. and Nehring, Anne and Höche, Nicole and Sweredoski, Michael J. and Pielot, Rainer and Hess, Sonja and Tirrell, David A. and Dieterich, Daniela C. and Schuman, Erin M. (2012) Dopaminergic modulation of the hippocampal neuropil proteome identified by bioorthogonal noncanonical amino acid tagging (BONCAT). Proteomics, 12 (15-16). pp. 2464-2476. ISSN 1615-9853. PMCID PMC3752339. https://resolver.caltech.edu/CaltechAUTHORS:20121005-102359212

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Abstract

Local protein synthesis and its activity-dependent modulation via dopamine receptor stimulation play an important role in synaptic plasticity – allowing synapses to respond dynamically to changes in their activity patterns. We describe here the metabolic labeling, enrichment, and MS-based identification of candidate proteins specifically translated in intact hippocampal neuropil sections upon treatment with the selective D1/D5 receptor agonist SKF81297. Using the noncanonical amino acid azidohomoalanine and click chemistry, we identified over 300 newly synthesized proteins specific to dendrites and axons. Candidates specific for the SKF81297-treated samples were predominantly involved in protein synthesis and synapse-specific functions. Furthermore, we demonstrate a dendrite-specific increase in proteins synthesis upon application of SKF81297. This study provides the first snapshot in the dynamics of the dopaminergic hippocampal neuropil proteome.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1002/pmic.201200112 DOIArticle
http://onlinelibrary.wiley.com/doi/10.1002/pmic.201200112/abstractPublisherArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3752339/PubMed CentralArticle
ORCID:
AuthorORCID
Sweredoski, Michael J.0000-0003-0878-3831
Hess, Sonja0000-0002-5904-9816
Tirrell, David A.0000-0003-3175-4596
Schuman, Erin M.0000-0002-7053-1005
Additional Information:© 2012 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim. Received: March 11, 2012. Revised: May 21, 2012. Accepted: May 22, 2012. Article first published online: 20 Aug. 2012. This work was supported by the German Academy for Natural Scientists Leopoldina and the DFG (Emmy Noether Program and a DIP grant to D. C. D.), the NIH (E. M. S. and D. A. T. 5R01GM62523–9), HHMI (E. M. S.), Gordon and Betty Moore Foundation (M. J. S., S. H.), and the Beckman Institute (M. J. S, S. H.).
Funders:
Funding AgencyGrant Number
German Academy for Natural Scientists LeopoldinaUNSPECIFIED
Deutsche Forschungsgemeinschaft (DFG)UNSPECIFIED
NIH5R01GM62523-9
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
Gordon and Betty Moore FoundationUNSPECIFIED
Caltech Beckman InstituteUNSPECIFIED
Subject Keywords:Animal proteomics; BONCAT; Click chemistry; Dopaminergic signaling; Local protein synthesis; Metabolic labeling; MUDPIT
Issue or Number:15-16
PubMed Central ID:PMC3752339
Record Number:CaltechAUTHORS:20121005-102359212
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20121005-102359212
Official Citation:Hodas, J. J. L., Nehring, A., Höche, N., Sweredoski, M. J., Pielot, R., Hess, S., Tirrell, D. A., Dieterich, D. C. and Schuman, E. M. (2012), Dopaminergic modulation of the hippocampal neuropil proteome identified by bioorthogonal noncanonical amino acid tagging (BONCAT). Proteomics, 12: 2464–2476. doi: 10.1002/pmic.201200112
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:34705
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:10 Oct 2012 18:23
Last Modified:09 Mar 2020 13:19

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