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A Novel Approach for Subretinal Implantation of Ultrathin Substrates Containing Stem Cell-Derived Retinal Pigment Epithelium Monolayer

Hu, Yuntao and Liu, Laura and Lu, Bo and Zhu, Danhong and Ribeiro, Ramiro and Diniz, Bruno and Thomas, Padmaja B. and Ahuja, Ashish K. and Hinton, David R. and Tai, Yu-Chong and Hikita, Sherry T. and Johnson, Lincoln V. and Clegg, Dennis O. and Thomas, Biju B. and Humayun, Mark S. (2012) A Novel Approach for Subretinal Implantation of Ultrathin Substrates Containing Stem Cell-Derived Retinal Pigment Epithelium Monolayer. Ophthalmic Research, 48 (4). pp. 186-191. ISSN 0030-3747. https://resolver.caltech.edu/CaltechAUTHORS:20121009-144517302

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Abstract

Objective: To evaluate the feasibility of a new technique for the implantation of ultrathin substrates containing stem cell-derived retinal pigment epithelium (RPE) cells into the subretinal space of retina-degenerate Royal College of Surgeon (RCS) rats. Methods: A platform device was used for the implantation of 4-µm-thick parylene substrates containing a monolayer of human embryonic stem cell-derived RPE (hESC-RPE). Normal Copenhagen rats (n = 6) and RCS rats (n = 5) were used for the study. Spectral-domain optical coherence tomography (SD-OCT) scanning and histological examinations were performed to confirm placement location of the implant. hESC-RPE cells attached to the substrate before and after implantation were evaluated using standard cell counting techniques. Results: SD-OCT scanning and histological examination revealed that the substrates were precisely placed in the rat's subretinal space. The hESC-RPE cell monolayer that covered the surface of the substrate was found to be intact after implantation. Cell counting data showed that less than 2% of cells were lost from the substrate due to the implantation procedure (preimplantation count 2,792 ± 74.09 cells versus postimplantation count 2,741 ± 62.08 cells). Detailed microscopic examination suggested that the cell loss occurred mostly along the edges of the implant. Conclusion: With the help of this platform device, it is possible to implant ultrathin substrates containing an RPE monolayer into the rat's subretinal space. This technique can be a useful approach for stem cell-based tissue bioengineering techniques in retinal transplantation research.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1159/000338749DOIUNSPECIFIED
http://www.ncbi.nlm.nih.gov/pubmed/22868580PubMed CentralUNSPECIFIED
ORCID:
AuthorORCID
Tai, Yu-Chong0000-0001-8529-106X
Humayun, Mark S.0000-0002-5830-5208
Additional Information:© 2012 S. Karger AG, Basel. Received: March 14, 2012. Accepted: March 21, 2012. Published online: August 3, 2012. We wish to thank our colleagues Zhenhai Chen. Xiaopeng Wang, Rongjuan Wu, Gilberto Raul Lopez, Juan Carlos Gutierrez, Juan Carlos Martinez and Rodrigo Brant, who made significant contributions to this research, and Gerald J. Chader and Susan Clarke for manuscript editing. This research was supported by CIRM DRT-01444, Research to Prevent Blindness and NEI gram EY03040.
Funders:
Funding AgencyGrant Number
CIRMDRI-01444
Research to Prevent BlindnessUNSPECIFIED
NEIEY03040
Subject Keywords:Transplantation; Stem cells; Parylene; Retinal pigment epithelium; Royal College of Surgeon rats
Other Numbering System:
Other Numbering System NameOther Numbering System ID
PubMed ID22868580
Issue or Number:4
Record Number:CaltechAUTHORS:20121009-144517302
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20121009-144517302
Official Citation:A Novel Approach for Subretinal Implantation of Ultrathin Substrates Containing Stem Cell-Derived Retinal Pigment Epithelium Monolayer. Hu Y, Liu L, Lu B, Zhu D, Ribeiro R, Diniz B, Thomas PB, Ahuja AK, Hinton DR, Tai YC, Hikita ST, Johnson LV, Clegg DO, Thomas BB, Humayun MS. Ophthalmic Res. 2012 Aug 3;48(4):186-191.
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:34799
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:10 Oct 2012 15:54
Last Modified:09 Mar 2020 13:18

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