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Crystallization and preliminary crystallographic studies of FoxE from Rhodobacter ferrooxidans SW2, an FeII oxidoreductase involved in photoferrotrophy

Pereira, L. and Saraiva, I. H. and Coelho, R. and Newman, D. K. and Louro, R. O. and Frazão, C. (2012) Crystallization and preliminary crystallographic studies of FoxE from Rhodobacter ferrooxidans SW2, an FeII oxidoreductase involved in photoferrotrophy. Acta Crystallographica. Section F, Structural Biology and Crystallization Communications, 68 (9). pp. 1106-1108. ISSN 1744-3091. PMCID PMC3433209. http://resolver.caltech.edu/CaltechAUTHORS:20121018-154043571

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Abstract

FoxE is a protein encoded by the foxEYZ operon of Rhodobacter ferrooxidans SW2 that is involved in Fe^II-based anoxygenic photosynthesis (`photoferrotrophy'). It is thought to reside in the periplasm, where it stimulates light-dependent Fe^II oxidation. It contains 259 residues, including two haem c-binding motifs. As no three-dimensional model is available and there is no structure with a similar sequence, crystals of FoxE were produced. They diffracted to 2.44 Å resolution using synchrotron radiation at the Fe edge. The phase problem was solved by SAD using SHELXC/D/E and the experimental maps confirmed the presence of two haems per molecule.


Item Type:Article
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http://dx.doi.org/10.1107/S174430911203271XDOIArticle
http://scripts.iucr.org/cgi-bin/paper?S174430911203271XPublisherArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3433209/PubMed CentralArticle
ORCID:
AuthorORCID
Newman, D. K.0000-0003-1647-1918
Additional Information:© International Union of Crystallography. Received 22 June 2012; accepted 18 July 2012. The authors gratefully acknowledge the ESRF, Grenoble, France for provision of synchrotron radiation and thank Susana Gonçalves, ITQB–UNL, Portugal for diffraction data collection. G. M. Sheldrick is thanked for valuable discussions and for providing the SHELXE program. This work was funded by projects MIT-Pt/BS-BB/1014/2008 from the MIT-Portugal Program and PTDC/EBB-BIO/098352/2008 from FCT, Portugal. IHS is the recipient of a PhD grant from FCT (SFRH/BD/36582/2007). DKN is an HHMI Investigator.
Funders:
Funding AgencyGrant Number
MIT-Portugal ProgramMIT-Pt/BS-BB/1014/2008
Fundação para a Ciência e a Tecnologia (FCT)PTDC/EBB-BIO/098352/2008
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
Subject Keywords:FoxE; iron oxidoreductases; Rhodobacter ferrooxidans SW2; photoferrotrophy
PubMed Central ID:PMC3433209
Record Number:CaltechAUTHORS:20121018-154043571
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20121018-154043571
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:34983
Collection:CaltechAUTHORS
Deposited By: Jason Perez
Deposited On:18 Oct 2012 23:39
Last Modified:24 Oct 2017 16:16

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