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Identification and characterization of homeobox transcription factor genes in Strongylocentrotus purpuratus, and their expression in embryonic development

Howard-Ashby, Meredith and Materna, Stefan C. and Brown, C. Titus and Chen, Lili and Cameron, R. Andrew and Davidson, Eric H. (2006) Identification and characterization of homeobox transcription factor genes in Strongylocentrotus purpuratus, and their expression in embryonic development. Developmental Biology, 300 (1). pp. 74-89. ISSN 0012-1606. https://resolver.caltech.edu/CaltechAUTHORS:20121031-074745343

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Abstract

A set of 96 homeobox transcription factors was identified in the Strongylocentrotus purpuratus genome using permissive blast searches with a large collection of authentic homeodomain sequences from mouse, human and fly. A phylogenetic tree was constructed to compare the sea urchin homeobox gene family to those of vertebrates, with the result that with the only a few exceptions, orthologs of all vertebrate homeodomain genes were uncovered by our search. QPCR time course measurements revealed that 65% of these genes are expressed within the first 48 h of development (late gastrula). For genes displaying sufficiently high levels of transcript during the first 24 h of development (late blastula), whole mount in situ hybridization was carried out up to 48 h to determine spatial patterns of expression. The results demonstrate that homeodomain transcription factors participate in multiple and diverse developmental functions, in that they are used at a range of time points and in every territory of the developing embryo.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/j.ydbio.2006.08.039DOIArticle
http://www.sciencedirect.com/science/article/pii/S0012160606010980PublisherArticle
ORCID:
AuthorORCID
Brown, C. Titus0000-0001-6001-2677
Cameron, R. Andrew0000-0003-3947-6041
Additional Information:© 2006 Elsevier Inc. Received for publication 18 April 2006; revised 4 August 2006; accepted 16 August 2006. Available online 22 August 2006. The authors would like to acknowledge Rachel Gray for her assistance in conducting the whole mount in situ hybridizations reported in the paper, and Deanna Thomas for her invaluable help with figures. This research was supported by NIH grant HD37105 and by the Office of Science (BER), US Department of Energy, grant DE-FG02-03ER63584.
Funders:
Funding AgencyGrant Number
NIHHD37105
Department of Energy (DOE)DE-FG02-03ER63584
Subject Keywords:Transcription factor; Sea urchin; Homeobox; Development
Issue or Number:1
Record Number:CaltechAUTHORS:20121031-074745343
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20121031-074745343
Official Citation:Meredith Howard-Ashby, Stefan C. Materna, C. Titus Brown, Lili Chen, R. Andrew Cameron, Eric H. Davidson, Identification and characterization of homeobox transcription factor genes in Strongylocentrotus purpuratus, and their expression in embryonic development, Developmental Biology, Volume 300, Issue 1, 1 December 2006, Pages 74-89, ISSN 0012-1606, 10.1016/j.ydbio.2006.08.039. (http://www.sciencedirect.com/science/article/pii/S0012160606010980)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:35193
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:31 Oct 2012 15:03
Last Modified:03 Oct 2019 04:26

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