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Gene families encoding transcription factors expressed in early development of Strongylocentrotus purpuratus

Howard-Ashby, Meredith and Materna, Stefan C. and Brown, C. Titus and Chen, Lili and Cameron, R. Andrew and Davidson, Eric H. (2006) Gene families encoding transcription factors expressed in early development of Strongylocentrotus purpuratus. Developmental Biology, 300 (1). pp. 90-107. ISSN 0012-1606. https://resolver.caltech.edu/CaltechAUTHORS:20121031-075952299

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Abstract

All genes encoding transcription factors of the bHLH, Nuclear Receptor, Basic Leucine Zipper, T-box, Smad, Sox, and other smaller families were identified in the Strongylocentrotus purpuratus genome by means of a permissive blast search of the genome using a database of known transcription factors. Phylogenetic trees were constructed for the major families, permitting a comparison of the regulatory protein repertoire of the sea urchin and other species. QPCR and whole mount in situ hybridization experiments revealed the temporal and spatial expression patterns of these genes during early development. These regulatory genes are initially expressed at a broad range of time points, and the large majority of genes of all families are expressed within the first 48 h of development. The observations suggest assignment of many regulatory genes to specific developmental sub-networks, including endomesodermal, oral, aboral, and apical.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/j.ydbio.2006.08.033 DOIArticle
http://www.sciencedirect.com/science/article/pii/S0012160606011031PublisherArticle
ORCID:
AuthorORCID
Brown, C. Titus0000-0001-6001-2677
Cameron, R. Andrew0000-0003-3947-6041
Additional Information:© 2006 Elsevier Inc. Received for publication 1 June 2006; revised 5 August 2006; accepted 16 August 2006. Available online 22 August 2006. The authors would like to acknowledge Rachel Gray for her invaluable assistance in performing in situs; Jongmin Nam for advice and assistance in generating phylogenetic trees; Jed Goldstone and Mark Hahn for their advice on nuclear receptor phylogeny; and Deanna Thomas for her assistance with figures.
Subject Keywords:Transcription factor; Sea urchin; Development; bHLH; Sox; Nuclear receptor; Basic zipper; smad
Issue or Number:1
Record Number:CaltechAUTHORS:20121031-075952299
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20121031-075952299
Official Citation:Meredith Howard-Ashby, Stefan C. Materna, C. Titus Brown, Lili Chen, R. Andrew Cameron, Eric H. Davidson, Gene families encoding transcription factors expressed in early development of Strongylocentrotus purpuratus, Developmental Biology, Volume 300, Issue 1, 1 December 2006, Pages 90-107, ISSN 0012-1606, 10.1016/j.ydbio.2006.08.033. (http://www.sciencedirect.com/science/article/pii/S0012160606011031)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:35194
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:31 Oct 2012 15:16
Last Modified:03 Oct 2019 04:26

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