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The tight junction protein claudin-1 influences cranial neural crest cell emigration

Fishwick, Katherine J. and Neiderer, Theresa E. and Jhingory, Sharon and Bronner, Marianne E. and Taneyhill, Lisa A. (2012) The tight junction protein claudin-1 influences cranial neural crest cell emigration. Mechanisms of Development, 129 (9-12). pp. 275-283. ISSN 0925-4773. PMCID PMC3482127. https://resolver.caltech.edu/CaltechAUTHORS:20121109-094813120

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Abstract

The neural crest is a population of migratory cells that follows specific pathways during development, eventually differentiating to form parts of the face, heart, and peripheral nervous system, the latter of which includes contributions from placodal cells derived from the ectoderm. Stationary, premigratory neural crest cells acquire the capacity to migrate by undergoing an epithelial-to-mesenchymal transition that facilitates their emigration from the dorsal neural tube. This emigration involves, in part, the dismantling of cell-cell junctions, including apically localized tight junctions in the neuroepithelium. In this study, we have characterized the role of the transmembrane tight junction protein claudin-1 during neural crest and placode ontogeny. Our data indicate that claudin-1 is highly expressed in the developing neuroepithelium but is down-regulated in migratory neural crest cells, although expression persists in the ectoderm from which the placode cells arise. Depletion or overexpression of claudin-1 augments or reduces neural crest cell emigration, respectively, but does not impact the development of several cranial placodes. Taken together, our results reveal a novel function for a tight junction protein in the formation of migratory cranial neural crest cells in the developing vertebrate embryo.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/j.mod.2012.06.006 DOIArticle
http://www.sciencedirect.com/science/article/pii/S0925477312000597PublisherArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3482127/PubMed CentralArticle
ORCID:
AuthorORCID
Bronner, Marianne E.0000-0003-4274-1862
Additional Information:© 2012 Elsevier Ireland Ltd. Received 3 April 2012; Received in revised form 5 June 2012; Accepted 26 June 2012; Available online 3 July 2012. The authors would like to thank Ms. Abigail Figat for technical assistance. This work was supported by Grants NIH-HD037105 and DE16459 (M.E.B.) and NSF IOS-0948525 (L.A.T.). Additional support for this research was provided by the University of Maryland from the Howard Hughes Medical Institute Undergraduate Science Education Program (T.E.N.).
Funders:
Funding AgencyGrant Number
NIHHD037105
NIHDE16459
NSFIOS-0948525
University of MarylandUNSPECIFIED
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
Subject Keywords:Claudin-1; Tight junctions; Neural crest; Placodes; EMT
Issue or Number:9-12
PubMed Central ID:PMC3482127
Record Number:CaltechAUTHORS:20121109-094813120
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20121109-094813120
Official Citation:Katherine J. Fishwick, Theresa E. Neiderer, Sharon Jhingory, Marianne E. Bronner, Lisa A. Taneyhill, The tight junction protein claudin-1 influences cranial neural crest cell emigration, Mechanisms of Development, Volume 129, Issues 9–12, September–December 2012, Pages 275-283, ISSN 0925-4773, 10.1016/j.mod.2012.06.006.
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:35378
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:09 Nov 2012 22:57
Last Modified:03 Oct 2019 04:27

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