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State-Selective Metabolic Labeling of Cellular Proteins

Ngo, John T. and Babin, Brett M. and Champion, Julie A. and Schuman, Erin M. and Tirrell, David A. (2012) State-Selective Metabolic Labeling of Cellular Proteins. ACS Chemical Biology, 7 (8). pp. 1326-1330. ISSN 1554-8929. PMCID PMC3423470. https://resolver.caltech.edu/CaltechAUTHORS:20121115-132451309

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Abstract

Transcriptional activity from a specified promoter can provide a useful marker for the physiological state of a cell. Here we introduce a method for selective tagging of proteins made in cells in which specified promoters are active. Tagged proteins can be modified with affinity reagents for enrichment or with fluorescent dyes for visualization. The method allows state-selective analysis of the proteome, whereby proteins synthesized in predetermined physiological states can be identified. The approach is demonstrated by proteome-wide labeling of bacterial proteins upon activation of the P_(BAD) promoter and the SoxRS regulon and provides a basis for analysis of more complex systems including spatially heterogeneous microbial cultures and biofilms.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1021/cb300238w DOIArticle
http://pubs.acs.org/doi/abs/10.1021/cb300238wPublisherArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3423470/PubMed CentralArticle
ORCID:
AuthorORCID
Babin, Brett M.0000-0002-4133-6665
Schuman, Erin M.0000-0002-7053-1005
Tirrell, David A.0000-0003-3175-4596
Additional Information:© 2012 American Chemical Society. Received: May 14, 2012. Accepted: June 7, 2012. Published: June 12, 2012. This work was supported by National Institutes of Health grant NIH R01 GM062523 and by the Institute for Collaborative Biotechnologies through grant W911NF-09-0001 from the U.S. Army Research Office.
Funders:
Funding AgencyGrant Number
NIHR01 GM062523
Army Research Office (ARO)W911NF-09-0001
Issue or Number:8
PubMed Central ID:PMC3423470
Record Number:CaltechAUTHORS:20121115-132451309
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20121115-132451309
Official Citation:State-Selective Metabolic Labeling of Cellular Proteins John T. Ngo, Brett M. Babin, Julie A. Champion, Erin M. Schuman, and David A. Tirrell ACS Chemical Biology20127 (8), 1326-1330
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:35490
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:20 Nov 2012 17:53
Last Modified:09 Mar 2020 13:19

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