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An anatomically comprehensive atlas of the adult human brain transcriptome

Hawrylycz, Michael J. and Koch, Christof (2012) An anatomically comprehensive atlas of the adult human brain transcriptome. Nature, 489 (7416). pp. 391-399. ISSN 0028-0836. PMCID PMC4243026.

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Neuroanatomically precise, genome-wide maps of transcript distributions are critical resources to complement genomic sequence data and to correlate functional and genetic brain architecture. Here we describe the generation and analysis of a transcriptional atlas of the adult human brain, comprising extensive histological analysis and comprehensive microarray profiling of ~900 neuroanatomically precise subdivisions in two individuals. Transcriptional regulation varies enormously by anatomical location, with different regions and their constituent cell types displaying robust molecular signatures that are highly conserved between individuals. Analysis of differential gene expression and gene co-expression relationships demonstrates that brain-wide variation strongly reflects the distributions of major cell classes such as neurons, oligodendrocytes, astrocytes and microglia. Local neighbourhood relationships between fine anatomical subdivisions are associated with discrete neuronal subtypes and genes involved with synaptic transmission. The neocortex displays a relatively homogeneous transcriptional pattern, but with distinct features associated selectively with primary sensorimotor cortices and with enriched frontal lobe expression. Notably, the spatial topography of the neocortex is strongly reflected in its molecular topography—the closer two cortical regions, the more similar their transcriptomes. This freely accessible online data resource forms a high-resolution transcriptional baseline for neurogenetic studies of normal and abnormal human brain function.

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Koch, Christof0000-0001-6482-8067
Additional Information:© 2012 Macmillan Publishers Limited. Received 22 December 2011; accepted 9 July 2012. We wish to thank the Allen Institute founders, P. G. Allen and J. Allen, for their vision, encouragement, and support. We express our gratitude to past and present Allen Institute staff members R. Adams, K. Aiona, A. Alpisa, J. Arnold, C. Bennet, K. Brouner, S. Butler, E. Byrnes, S. Caldejon, J. Campiche, A. Carey, J. Chen, C. Copeland, C. Cuhaciyan, T. Desta, N. Dotson, S. Faber, T. Fliss, E. Fulfs, G. Gee, T. Gilbert, L. Gourley, G. Gu, J. Heilman, N. Ivanov, K. Keyser, A. Kriedberg, J. Laoenkue, F. Lee, S. Levine, L. Luong, N. Mastan, N. Mosqueda, E. Mott, N. Motz, D. Muzia, K. Ngo, A. Oldre, E. Olson, J. Parente, J. Phillips, L. Potekhina, T. Roberts, K. Roll, D. Rosen, M. Sarreal, S. Shapouri, N. Shapovalova, C. Simpson, D. Simpson, M. Smith, N. Stewart, K. Sweeney, A. Szafer, L. Velasquez, U. Wagley, W. Wakeman, C. White and B. Youngstrom for their technical assistance. We thank C. Long for mechanical engineering contract work. We thank R. Gullapalli, A. McMillan and R. Morales for post-mortem magnetic resonance imaging and radiology interpretation of MR data; J. Cottrell, M. Davis, R. Johnson, K.Moraniec, R. Vigorito, A. Weldon and the NICHD Brain and Tissue Bank for Developmental Disorders for tissue acquisition and processing; J. Davis for donor coordination; F. Mamdani, M. Martin, E. Moon, L. Morgan, B. Rollins and D. Walsh for tissue processing and psychological autopsy (DW); D. Patel for magnetic resonance imaging; and J. Sonnen for consultation on tissue microneuropathology. We also thank the External RNA Controls Consortium (ERCC), the USNational Institute of Standards (NIST) and Technology, and M. Salit for access to ERCC transcripts during Phase V testing.Weare grateful to Beckman Coulter Genomics (formerly Cogenics) and their staff P. Hurban, E. Lobenhofer, K. Phillips, A. Rouse and S. Beaver for microarray data generation and design of the custom Agilent array. We also wish to thank the Allen Human Brain Atlas Advisory Council members D. Geschwind, R. Gibbs, P. Hof, E. Jones, C. Koch, C. Saper, L. Swanson, A. Toga and D. Van Essen for their scientific guidance and dedication to the successful execution of this project. The project described was supported in part by Grant Numbers 1C76HF15069-01-00 and 1 1C76HF19619-01-00 from the Department of Health and Human Services Health Resources and Services Administration Awards and its contents are solely the responsibility of the authors and do not necessarily represent the official views of the Department of Health and Human Services Health Resources and Services Administration Awards. S.G.N.G and L.V.L. were supported by the MRCD, Wellcome Trust and European Union Seventh Framework Programme under grants 241498 EUROSPIN, 242167 SynSys, and 241995 GENCODYS Projects. Author Contributions A.R.J., A.L.G.-B., E.H.S. and K.A.S. contributed significantly to overall project design. A.L.G.-B., E.H.S., K.A.S., A.E. and P.W. managed the tissue and sample processing in the laboratory. D.B., A.F.B., R.A.D., J.G., B.W.G., R.E.H., M.K., T.A.L., P.D.P., S.E.P., M.R., J.J.R. and B.E.S. contributed to tissue and sample processing. E.H.S. and Z.L.R. contributed to establishing the tissue acquisition pipeline. P.M.C., B.D.D., D.R.F., L.L., P.A.S., M.P.V. and H.R.Z. contributed to tissue acquisition and MR imaging. Z.L.R., A.B., M.M.C., N.D., A.J., J.M.J., E.T.L., S.C.S. and P.R.H. contributed to protocol development. S.D., J.M.J., C.R.S. and D.W. provided engineering support. A.L.G.-B., R.A.D., P.D.P., J.G.H., J.A.Mo., J.J.R. and B.E.S. contributed to the neuroanatomical design and implementation. L.N. and C.D. managed the creation of the data pipeline, visualization and mining tools. L.N., C.D. and C.C.O. contributed to the overall online product concept. L.N., C.A., M.C., J.C., T.A.D., D.F., Z.H., C.La., Y.L. and A.J.S. contributed to the creation of the data pipeline, visualization and mining tools. M.J.H., E.S.L., J.A.Mi., D.H.G., L.N.L., C.F.B., S.M.Sm., S.G.N.G., A.LG.-.B., E.H.S., K.A.S., A.B., D.B., V.F., J.G., D.R.H., S.H., C.Le., J.S., S.M.Su., P.R.H. and C.K. contributed to data analysis and interpretation. A.R.J. supervised the overall project, and themanuscriptwas writtenby M.J.H. and E.S.L. with input from other authors.
Group:Koch Laboratory (KLAB)
Funding AgencyGrant Number
Department of Health and Human Services Health Resources and Services Administration1C76HF15069-01-00
Department of Health and Human Services Health Resources and Services Administration1 1C76HF19619-01-00
European Research Council (ERC)241498 EUROSPIN
European Research Council (ERC)242167 SynSys
European Research Council (ERC)241995 GENCODYS
Subject Keywords:Neuroscience; Genetics; Genomics; Databases
Issue or Number:7416
PubMed Central ID:PMC4243026
Record Number:CaltechAUTHORS:20121127-084920112
Persistent URL:
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:35657
Deposited By: Jason Perez
Deposited On:27 Nov 2012 22:34
Last Modified:03 Oct 2019 04:30

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