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Application of a new microcantilever biosensor resonating at the air–liquid interface for direct insulin detection and continuous monitoring of enzymatic reactions

Park, Jungwook and Karsten, Stanislav L. and Nishida, Shuhei and Kawakatsu, Hideki and Fujita, Hiroyuki (2012) Application of a new microcantilever biosensor resonating at the air–liquid interface for direct insulin detection and continuous monitoring of enzymatic reactions. Lab on a Chip, 12 (20). pp. 4115-4119. ISSN 1473-0197. https://resolver.caltech.edu/CaltechAUTHORS:20121204-110240639

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Abstract

Here we describe the application of a recently developed high-resolution microcantilever biosensor resonating at the air–liquid interface for the continuous detection of antigen–antibody and enzyme–substrate interactions. The cantilever at the air–liquid interface demonstrated 50% higher quality factor and a 5.7-fold increase in signal-to-noise-ratio (SNR) compared with one immersed in the purified water. First, a label-free detection of a low molecular weight protein (insulin, 5.8 kDa) in physiological concentration was demonstrated. The liquid facing side of the cantilever was functionalized by coating its surface with insulin antibodies, while the opposite side was exposed to air. The meniscus membrane at the micro-slit around the cantilever sustained the liquid in the microchannel. After optimizing the process of surface functionalization, the resonance frequency shift was successfully measured for insulin solutions of 0.4, 2.0, and 6.3 ng ml^(−1). To demonstrate additional application of the device for monitoring enzymatic protein degradation, the liquid facing microcantilever surface was coated with human recombinant SOD1 (superoxide dismutase 1) and exposed to various concentrations of proteinase K solution, and the kinetics of the SOD1 digestion was continuously monitored. The results showed that it is a suitable tool for sensitive protein detection and analysis.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1039/C2LC40232GDOIUNSPECIFIED
http://pubs.rsc.org/en/Content/ArticleLanding/2012/LC/c2lc40232gPublisherUNSPECIFIED
Additional Information:© 2012 Royal Society of Chemistry. Received 06 Mar 2012, Accepted 03 Jul 2012. First published on the web 06 Jul 2012. This work was supported by the research project Development of a bio/nano-hybrid platform technology towards regenerative medicine (project leader: Prof. Hidetoshi Kotera) of CREST in Japan Science and Technology Agency (JST). Jungwook Park received support from G-COE program on "Secure-life Electronics" of Ministry of Education, Culture, Sports, Science and Technology (MEXT), and Stanislav L. Karsten received support from Japan Society for the Promotion of Science (JSPS).
Funders:
Funding AgencyGrant Number
Japan Science and Technology Agency (JST) Core Research for Evolutional Science and Technology (CREST)UNSPECIFIED
Ministry of Education, Culture, Sports, Science and Technology (MEXT)UNSPECIFIED
Japan Society for the Promotion of Science (JSPS)UNSPECIFIED
Issue or Number:20
Record Number:CaltechAUTHORS:20121204-110240639
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20121204-110240639
Official Citation:Application of a new microcantilever biosensor resonating at the air–liquid interface for direct insulin detection and continuous monitoring of enzymatic reactions Jungwook Park, Stanislav L. Karsten, Shuhei Nishida, Hideki Kawakatsu and Hiroyuki Fujita Lab Chip, 2012, 12, 4115-4119 DOI: 10.1039/C2LC40232G
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:35790
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:04 Dec 2012 19:26
Last Modified:03 Oct 2019 04:31

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