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Molecular and Cellular Approaches for Diversifying and Extending Optogenetics

Gradinaru, Viviana and Zhang, Feng and Ramakrishnan, Charu and Mattis, Joanna and Prakash, Rohit and Diester, Ilka and Goshen, Inbal and Thompson, Kimberly R. and Deisseroth, Karl (2010) Molecular and Cellular Approaches for Diversifying and Extending Optogenetics. Cell, 141 (1). pp. 154-165. ISSN 0092-8674. PMCID PMC4160532.

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Optogenetic technologies employ light to control biological processes within targeted cells in vivo with high temporal precision. Here, we show that application of molecular trafficking principles can expand the optogenetic repertoire along several long-sought dimensions. Subcellular and transcellular trafficking strategies now permit (1) optical regulation at the far-red/infrared border and extension of optogenetic control across the entire visible spectrum, (2) increased potency of optical inhibition without increased light power requirement (nanoampere-scale chloride-mediated photocurrents that maintain the light sensitivity and reversible, step-like kinetic stability of earlier tools), and (3) generalizable strategies for targeting cells based not only on genetic identity, but also on morphology and tissue topology, to allow versatile targeting when promoters are not known or in genetically intractable organisms. Together, these results illustrate use of cell-biological principles to enable expansion of the versatile fast optogenetic technologies suitable for intact-systems biology and behavior.

Item Type:Article
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URLURL TypeDescription DOIArticle CentralArticle
Gradinaru, Viviana0000-0001-5868-348X
Additional Information:© 2010 Elsevier Inc. Received: October 9, 2009; Revised: January 11, 2010; Accepted: February 18, 2010; Published online: March 18, 2010. V.G. is supported by the Stanford Interdisciplinary Graduate Fellowship, F.Z. by the National Institutes of Health (NIH) and Harvard Society of Fellows, J.M. by the Stanford Medical Scientist Training Program, R.P. by the NIH, I.D. by the German Academic Exchange Service and the Human Frontier Science Program, I.G. by the Machiah Foundation and the Weizmann Institute, K.T. by the National Alliance for Research on Schizophrenia and Depression, and K.D. by the Woo, Keck, Snyder, McKnight, Yu, and Coulter Foundations, as well as by the California Institute of Regenerative Medicine, the National Science Foundation, and the NIH. We thank the entire Deisseroth lab for their support.
Funding AgencyGrant Number
Stanford Interdisciplinary Graduate FellowshipUNSPECIFIED
Harvard Society of FellowsUNSPECIFIED
Stanford Medical Scientist Training ProgramUNSPECIFIED
Deutscher Akademischer Austauschdienst (DAAD)UNSPECIFIED
Machiah FoundationUNSPECIFIED
Weizmann InstituteUNSPECIFIED
National Alliance for Research on Schizophrenia and DepressionUNSPECIFIED
California Institute of Regenerative MedicineUNSPECIFIED
Human Frontier Science ProgramUNSPECIFIED
W. M. Keck FoundationUNSPECIFIED
McKnight FoundationUNSPECIFIED
H. L. Snyder Medical FoundationUNSPECIFIED
Albert Yu and Mary Bechmann FoundationUNSPECIFIED
Wallace Coulter FoundationUNSPECIFIED
Subject Keywords:Molneuro; Sysneuro
Issue or Number:1
PubMed Central ID:PMC4160532
Record Number:CaltechAUTHORS:20121206-083746393
Persistent URL:
Official Citation:Viviana Gradinaru, Feng Zhang, Charu Ramakrishnan, Joanna Mattis, Rohit Prakash, Ilka Diester, Inbal Goshen, Kimberly R. Thompson, Karl Deisseroth, Molecular and Cellular Approaches for Diversifying and Extending Optogenetics, Cell, Volume 141, Issue 1, 2 April 2010, Pages 154-165, ISSN 0092-8674, 10.1016/j.cell.2010.02.037.
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:35840
Deposited By: Tony Diaz
Deposited On:07 Dec 2012 22:11
Last Modified:03 Oct 2019 04:32

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