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Establishment of a set of inbred strains of the pine wood nematode, Bursaphelenchus xylophilus (Aphelenchida: Aphelenchoididae), and evidence of their varying levels of virulence

Shinya, Ryoji and Takeuchi, Yuko and Ichimura, Keita and Takemoto, Shuhei and Futai, Kazuyoshi (2012) Establishment of a set of inbred strains of the pine wood nematode, Bursaphelenchus xylophilus (Aphelenchida: Aphelenchoididae), and evidence of their varying levels of virulence. Applied Entomology and Zoology, 47 (4). pp. 341-350. ISSN 0003-6862. doi:10.1007/s13355-012-0124-8.

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Pine wilt disease (PWD) caused by the pine wood nematode, Bursaphelenchus xylophilus (Steiner and Buhrer) Nickle, has become a worldwide problem. The pathogenic mechanism of PWD continues to remain controversial, which in part may be attributed to the lack of universal materials of B. xylophilus with a high genetic purity. The intrinsic high genetic diversity in B. xylophilus isolates/populations must be a fatal obstacle for performing forward genetics and other molecular approaches to controlling them. We conducted a series of successive full-sib mating of conventional isolates of B. xylophilus to establish a set of inbred strains. Using DNA markers, we also determined their genetic diversity and biological characteristics, such as virulence and reproductive ability. Consequently, the newly established strains yielded a higher genetic purity than the conventional isolates and showed varying virulence despite sharing a common ancestor. The significance of this study lies not only in establishing a set of inbred strains of B. xylophilus with the certification of their purity but also in demonstrating that avirulent strain(s) with a genotype similar to the virulent strains can be obtained by simple successive full-sib mating. This technique is one of the most powerful tools for elucidating the pathogenic mechanism(s) of PWD.

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Additional Information:© 2012 The Japanese Society of Applied Entomology and Zoology. Received: 14 February 2012; Accepted: 9 July 2012; Published online: 29 July 2012. The authors thank Professor Dr. M.M. Mota, University of Évora, Portugal, and Dr. J.R. Sutherland, Canada, for helpful suggestions and linguistic correction of the manuscript. The authors also thank Professor Dr. M. Ueda, Kyoto University, Japan, for helpful suggestions regarding research plans. This study was supported by a Grant-in-Aid for JSPS Fellows and an Inamori Foundation.
Funding AgencyGrant Number
Japan Society for the Promotion of Science (JSPS) Grant-in-AidUNSPECIFIED
Inamori FoundationUNSPECIFIED
Subject Keywords:AFLP; Inbred strain; Full-sib mating; Reproductive ability; Virulence
Issue or Number:4
Record Number:CaltechAUTHORS:20121210-160720640
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:35897
Deposited By: Jason Perez
Deposited On:12 Dec 2012 00:16
Last Modified:09 Nov 2021 23:18

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