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Decreased Reactive Oxygen Species Production in Cells with Mitochondrial Haplogroups Associated with Longevity

Chen, Ai and Raule, Nicola and Chomyn, Anne and Attardi, Giuseppe (2012) Decreased Reactive Oxygen Species Production in Cells with Mitochondrial Haplogroups Associated with Longevity. PLoS ONE, 7 (10). Art. No. e46473. ISSN 1932-6203. PMCID PMC3483264. https://resolver.caltech.edu/CaltechAUTHORS:20121218-075633343

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Abstract

Mitochondrial DNA (mtDNA) is highly polymorphic, and its variations in humans may contribute to individual differences in function. Zhang and colleagues found a strikingly higher frequency of a C150T transition in the D-loop of mtDNA from centenarians and twins of an Italian population, and also demonstrated that this base substitution causes a remodeling of the mtDNA 151 replication origin in human leukocytes and fibroblasts [1]. The C150T transition is a polymorphism associated with several haplogroups. To determine whether haplogroups that carry the C150T transition display any phenotype that may be advantageous for longevity, we analyzed cybrids carrying or not the C150T transition. These cybrids were obtained by fusing cytoplasts derived from human fibroblasts with human mtDNA-less cells (ρ^0 cells). We chose for cybrid construction and analysis haplogroup-matched pairs of fibroblast strains containing or not the C150T transition. In particular, we used, as one pair of mtDNA donors, a fibroblast strain of the U3a haplogroup, carrying the C150T transition and a strain of the U-K2 haplogroup, without the C150T transition, and as another pair, fibroblasts of the J2b haplogroup, carrying the C150T transition and of the J1c haplogroup, without the C150T transition. We have found no association of respiratory capacity, mtDNA level, mitochondrial gene expression level, or growth rate with the presence of the C150T transition. However, we have found that the cybrids with haplogroups that include the C150T transition have in common a lower reactive oxygen species (ROS) production rate than the haplogroup-matched cybrids without that transition. Thus, the lower ROS production rate may be a factor in the increased longevity associated with the U and the J2 haplogroups. Of further interest, we found that cybrids with the U3a haplogroup exhibited a higher respiration rate than the other cybrids examined.


Item Type:Article
Related URLs:
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http://dx.doi.org/10.1371/journal.pone.0046473 DOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3483264/PubMed CentralArticle
Additional Information:© 2012 Chen et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Received December 13, 2011; Accepted August 31, 2012; Published October 29, 2012. Editor: Nagendra Yadava, UMASS-Amherst/Tufts University School of Medicine, United States of America. Funding: This work was support by National Institutes of Health grant R01 AG012117 and the Ellison Foundation Senior Scholar Award, both to Guiseppe Attardi. The Grace C. Steele Foundation also provided support. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. We are grateful to Jaehyoung Cho for help with the RT-PCR analysis of mtDNA level and for helpful discussions. We thank Eduardo Ruiz-Pesini and Min-Xin Guan for advice on haplogroup analysis and sequencing of mtDNA, respectively. Author Contributions: Conceived and designed the experiments: GA A. Chen A. Chomyn. Performed the experiments: A. Chen NR. Analyzed the data: A. Chen A. Chomyn NR. Wrote the paper: GA A. Chen A. Chomyn.
Funders:
Funding AgencyGrant Number
NIHR01 AG012117
Ellison Medical FoundationUNSPECIFIED
Grace C. Steele FoundationUNSPECIFIED
Issue or Number:10
PubMed Central ID:PMC3483264
Record Number:CaltechAUTHORS:20121218-075633343
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20121218-075633343
Official Citation:Chen A, Raule N, Chomyn A, Attardi G (2012) Decreased Reactive Oxygen Species Production in Cells with Mitochondrial Haplogroups Associated with Longevity. PLoS ONE 7(10): e46473. doi:10.1371/journal.pone.0046473
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:36019
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:18 Dec 2012 16:29
Last Modified:12 Feb 2020 00:24

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