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Assessment of lamellar level properties in mouse bone utilizing a novel spherical nanoindentation data analysis method

Pathak, Siddhartha and Vachhani, Shraddha J. and Jepsen, Karl J. and Goldman, Haviva M. and Kalidindi, Surya R. (2012) Assessment of lamellar level properties in mouse bone utilizing a novel spherical nanoindentation data analysis method. Journal of the Mechanical Behavior of Biomedical Materials, 13 . pp. 102-117. ISSN 1751-6161. doi:10.1016/j.jmbbm.2012.03.018.

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In this work, we demonstrate the viability of using our recently developed data analysis procedures for spherical nanoindentation in conjunction with Raman spectroscopy for studying lamellar-level correlations between the local composition and local mechanical properties in mouse bone. Our methodologies allow us to convert the raw load-displacement datasets to much more meaningful indentation stress–strain curves that accurately capture the loading and unloading elastic moduli, the indentation yield points, as well as the post-yield characteristics in the tested samples. Using samples of two different inbred mouse strains, A/J and C57BL/6J (B6), we successfully demonstrate the correlations between the mechanical information obtained from spherical nanoindentation measurements to the local composition measured using Raman spectroscopy. In particular, we observe that a higher mineral-to-matrix ratio correlated well with a higher local modulus and yield strength in all samples. Thus, new bone regions exhibited lower moduli and yield strengths compared to more mature bone. The B6 mice were also found to exhibit lower modulus and yield strength values compared to the more mineralized A/J strain.

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Additional Information:© 2012 Elsevier Ltd. Received 8 February 2012; Received in revised form 23 March 2012; Accepted 26 March 2012; Available online 11 May 2012. This work was supported by grants from the National Institutes of Health (NIH AR044927). SK and SJV acknowledge funding from ARO grant W911NF-10-1-0409. SP and SJV acknowledge support from the 2007 and 2011 Sigma Xi Grants-in-Aid of Research (GIAR) program for this work, respectively. SP also gratefully acknowledges support from theW.M. Keck Institute for Space Studies Postdoctoral Fellowship program during the writing of this manuscript. The MTS XPs nanoindentation system and the Renishaw 1000 Raman micro spectrometer used in this study are maintained and operated by the Centralized Research Facilities in the College of Engineering at Drexel University. The authors also wish to thank Dr. Phil Nasser and Dr. Hayden-William Courtland (both at Mount Sinai School of Medicine, New York, NY) for assistance with sample preparation, as well as Dr. Zhorro Nikolov and Ms Melanie Patel (Drexel University) for help with Raman measurements.
Group:Keck Institute for Space Studies
Funding AgencyGrant Number
Army Research Office (ARO)W911NF-10-1-0409
Sigma Xi Grants-in-Aid of Research (GIAR)UNSPECIFIED
W. M. Keck Institute for Space StudiesUNSPECIFIED
Subject Keywords:Bone; Nanoindentation; Raman spectroscopy
Record Number:CaltechAUTHORS:20130102-135402426
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Official Citation:Siddhartha Pathak, Shraddha J. Vachhani, Karl J. Jepsen, Haviva M. Goldman, Surya R. Kalidindi, Assessment of lamellar level properties in mouse bone utilizing a novel spherical nanoindentation data analysis method, Journal of the Mechanical Behavior of Biomedical Materials, Volume 13, September 2012, Pages 102-117, ISSN 1751-6161, 10.1016/j.jmbbm.2012.03.018.
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:36119
Deposited By: Tony Diaz
Deposited On:11 Feb 2013 23:29
Last Modified:09 Nov 2021 23:20

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