A Caltech Library Service

Quantitating Cell–Cell Interaction Functions with Applications to Glioblastoma Multiforme Cancer Cells

Wang, Jun and Tham, Douglas and Wei, Wei and Shin, Young Shik and Ma, Chao and Ahmad, Habib and Shi, Qihui and Yu, Jenkan and Levine, Raphael D. and Heath, James R. (2012) Quantitating Cell–Cell Interaction Functions with Applications to Glioblastoma Multiforme Cancer Cells. Nano Letters, 12 (12). pp. 6101-6106. ISSN 1530-6984. PMCID PMC3680341.

[img] PDF - Accepted Version
See Usage Policy.

PDF - Supplemental Material
See Usage Policy.


Use this Persistent URL to link to this item:


We report on a method for quantitating the distance dependence of cell–cell interactions. We employ a microchip design that permits a multiplex, quantitative protein assay from statistical numbers of cell pairs, as a function of cell separation, with a 0.15 nL volume microchamber. We interrogate interactions between pairs of model brain cancer cells by assaying for six functional proteins associated with PI3k signaling. At short incubation times, cells do not appear to influence each other, regardless of cell separation. For 6 h incubation times, the cells exert an inhibiting influence on each other at short separations and a predominately activating influence at large separation. Protein-specific cell–cell interaction functions are extracted, and by assuming pairwise additivity of those interactions, the functions are shown to correctly predict the results from three-cell experiments carried out under the identical conditions.

Item Type:Article
Related URLs:
URLURL TypeDescription DOIArticle CentralArticle
Wei, Wei0000-0002-1018-7708
Heath, James R.0000-0001-5356-4385
Additional Information:© 2012 American Chemical Society. Received: July 24, 2012; Revised: October 24, 2012; Published: November 6, 2012. This work was funded by the National Cancer Institute (5U54 CA119347: J.R.H. PI), the Ben and Catherine Ivy Foundation, the Grand Duchy of Luxembourg, and the Jean Perkins Foundation. We acknowledge very helpful discussions from Professor Paul Mischel.
Funding AgencyGrant Number
NIH5U54 CA119347
Ben and Catherine Ivy FoundationUNSPECIFIED
Grand Duchy of LuxembourgUNSPECIFIED
Jean Perkins FoundationUNSPECIFIED
Subject Keywords:glioblastoma multiforme; microfluidics; single cell proteomics; cell interactions
Issue or Number:12
PubMed Central ID:PMC3680341
Record Number:CaltechAUTHORS:20130103-153656327
Persistent URL:
Official Citation:Quantitating Cell–Cell Interaction Functions with Applications to Glioblastoma Multiforme Cancer Cells Jun Wang, Douglas Tham, Wei Wei, Young Shik Shin, Chao Ma, Habib Ahmad, Qihui Shi, Jenkan Yu, Raphael D. Levine, and James R. Heath Nano Letters201212 (12), 6101-6106
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:36157
Deposited By: Tony Diaz
Deposited On:07 Jan 2013 23:10
Last Modified:18 Dec 2019 23:36

Repository Staff Only: item control page