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Precise overgrowth composition during biomineral culture and inorganic precipitation

Gagnon, Alexander C. and DePaolo, Donald J. and Adkins, Jess F. (2012) Precise overgrowth composition during biomineral culture and inorganic precipitation. Chemical Geology, 330 . pp. 188-196. ISSN 0009-2541.

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We introduce a method to analyze element ratios and isotope ratios in mineral overgrowths. This general technique can quantify environmental controls on proxy behavior for a range of cultured biominerals and can also measure compositional effects during seeded mineral growth. Using a media enriched in multiple stable isotopes, the method requires neither the mass nor the composition of the initial seed or skeleton to be known and involves only bulk isotope measurements. By harnessing the stability and sensitivity of bulk analysis the new approach promises high precision measurements for a range of elements and isotopes. This list includes trace species and select non-traditional stable isotopes, systems where sensitivity and external reproducibility currently limit alternative approaches like secondary ion mass spectrometry (SIMS) and laser ablation mass spectrometry. Since the method separates isotopically labeled growth from unlabeled material, well-choreographed spikes can resolve the compositional effects of different events through time. Among other applications, this feature could be used to separate the impact of day and night on biomineral composition in organisms with photosymbionts.

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Adkins, Jess F.0000-0002-3174-5190
Additional Information:© 2012 Published by Elsevier B.V. Received 2 May 2012. Received in revised form 22 August 2012. Accepted 22 August 2012. Available online 31 August 2012. This work was supported in part by the Director, Office of Science, Office of Basic Energy Sciences, Chemical Sciences Geosciences and Bioscience Program of the U.S. Department of Energy under Contract No. DEAC02-05CH11231. ACG would like to thank Jonathan Erez for his encouragement and for thoughtful discussions; conversations that helped motivate this research. This manuscript benefited from constructive suggestions by two anonymous reviewers.
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Department of Energy (DOE)DE-AC02-05CH11231
Subject Keywords:Isotope geochemistry; Crystal growth; Biomineralization; Mass spectrometry; Metal/calcium; Trace metals
Record Number:CaltechAUTHORS:20130104-093434755
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Official Citation:Alexander C. Gagnon, Donald J. DePaolo, Jess F. Adkins, Precise overgrowth composition during biomineral culture and inorganic precipitation, Chemical Geology, Volumes 330–331, 10 November 2012, Pages 188-196, ISSN 0009-2541, 10.1016/j.chemgeo.2012.08.022. (
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:36163
Deposited By: Ruth Sustaita
Deposited On:04 Jan 2013 18:03
Last Modified:24 Feb 2020 10:30

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