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A calcium ion-selective electrode array for monitoring the activity of HepG2/C3As in a microchannel

Park, Jungwook and Meissner, Robert and Ducloux, Olivier and Renaud, Philippe and Fujita, Hiroyuki (2012) A calcium ion-selective electrode array for monitoring the activity of HepG2/C3As in a microchannel. Sensors and Actuators B: Chemical, 174 . pp. 473-477. ISSN 0925-4005. https://resolver.caltech.edu/CaltechAUTHORS:20130104-133019548

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Abstract

Variations in calcium ion (Ca^(2+)) concentrations play a crucial role in cell–cell communication; periodic changes act as cellular signals. In this study, we developed a solid-state calcium ion-selective electrode (Ca^(2+) ISE) integrated in a microchannel to measure extracellular Ca^(2+) variations; an ISE has a high detection sensitivity (as low as 10^(−9) M) and selectivity against potassium ions (K^+). The sensor was used to monitor the cellular activities of HepG2/C3As cultured in a microchannel. We measured a periodically oscillating extracellular Ca^(2+) concentration that was superimposed on a gradual increase in the Ca^(2+) concentration after potassium chloride (KCl) stimulation. The oscillation period was in the range of 30–50 s, which was in good agreement with the literature.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/j.snb.2012.07.098DOIUNSPECIFIED
http://www.sciencedirect.com/science/article/pii/S0925400512007915PublisherUNSPECIFIED
Additional Information:© 2012 Elsevier B.V. Received 10 April 2012. Received in revised form 26 July 2012. Accepted 26 July 2012. Available online 10 August 2012. This work was supported by the research project “Development of a bio/nano-hybrid platform technology toward regenerative medicine” (Project Leader: Prof. Hidetoshi Kotera) in Core Research for Evolutional Science and Technology (CREST) of the Japan Science and Technology Agency (JST) and by the International Training Program (ITP) of the Japan Society for the Promotion of Science (JSPS). One of the authors, Jungwook Park, was supported by the Global Center of Excellence (GCOE) Program “Secure-life Electronics” of the Ministry of Education, Culture, Sports, Science and Technology (MEXT).
Funders:
Funding AgencyGrant Number
Japan Science and Technology Agency (JST) Core Research for Evolutional Science and Technology (CREST)UNSPECIFIED
Japan Society for the Promotion of Science (JSPS) International Training Program (ITP)UNSPECIFIED
Global Center of Excellence (GCOE) Program “Secure-life Electronics”UNSPECIFIED
Subject Keywords:Potentiometer; Solid-state ISE; Calcium ion; Oscillation; HepG2/C3A
Record Number:CaltechAUTHORS:20130104-133019548
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20130104-133019548
Official Citation:Jungwook Park, Robert Meissner, Olivier Ducloux, Philippe Renaud, Hiroyuki Fujita, A calcium ion-selective electrode array for monitoring the activity of HepG2/C3As in a microchannel, Sensors and Actuators B: Chemical, Volume 174, November 2012, Pages 473-477, ISSN 0925-4005, 10.1016/j.snb.2012.07.098. (http://www.sciencedirect.com/science/article/pii/S0925400512007915)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:36174
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:04 Jan 2013 21:54
Last Modified:03 Oct 2019 04:35

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