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Regeneration in Arabidopsis Tissue Culture

Sugimoto, Kaoru and Meyerowitz, Elliot M. (2013) Regeneration in Arabidopsis Tissue Culture. In: Plant Organogenesis: Methods and Protocols. Methods in Molecular Biology. No.959. Springer , New York, pp. 265-275. ISBN 9781627032209.

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An entire Arabidopsis plant can be regenerated from a small piece of tissue by two sequential hormonal treatments in tissue culture. Currently this in vitro regeneration system is a good system to study the mechanism by which plants show regenerative plasticity. Also, it is useful to test the hormone sensitivity of plants and to propagate sterile lines in Arabidopsis. Here we describe a standard protocol for regenerating Arabidopsis plants in tissue culture, and for preparing and observing samples using confocal microscopy to study cells during regeneration.

Item Type:Book Section
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Meyerowitz, Elliot M.0000-0003-4798-5153
Additional Information:© 2013 Springer Science+Business Media. We thank Kazuhito Akama, Toshiro Ito, and Tatsuo Kakimoto for valuable information about tissue culture media formulations, Cory Tobin and Paul Tarr for information and technical suggestions about FM dye staining, and Carolyn Ohno for information about seeds sterilization. This work was supported by the US National Science Foundation (Grant IOS-0846192 to E.M.M.) and the Japan Society for the Promotion of Science (to K.S.).
Funding AgencyGrant Number
Japan Society for the Promotion of Science (JSPS)UNSPECIFIED
Subject Keywords:Arabidopsis thaliana , Plant regeneration , Tissue culture , Plant hormone , Callus formation , Shoot induction , Root induction
Series Name:Methods in Molecular Biology
Issue or Number:959
Record Number:CaltechAUTHORS:20130114-151324440
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:36364
Deposited By: Tony Diaz
Deposited On:16 Jan 2013 19:19
Last Modified:09 Nov 2021 23:21

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