CaltechAUTHORS
  A Caltech Library Service

Regeneration in Arabidopsis Tissue Culture

Sugimoto, Kaoru and Meyerowitz, Elliot M. (2013) Regeneration in Arabidopsis Tissue Culture. In: Plant Organogenesis: Methods and Protocols. Methods in Molecular Biology. No.959. Springer , New York, pp. 265-275. ISBN 9781627032209. https://resolver.caltech.edu/CaltechAUTHORS:20130114-151324440

Full text is not posted in this repository. Consult Related URLs below.

Use this Persistent URL to link to this item: https://resolver.caltech.edu/CaltechAUTHORS:20130114-151324440

Abstract

An entire Arabidopsis plant can be regenerated from a small piece of tissue by two sequential hormonal treatments in tissue culture. Currently this in vitro regeneration system is a good system to study the mechanism by which plants show regenerative plasticity. Also, it is useful to test the hormone sensitivity of plants and to propagate sterile lines in Arabidopsis. Here we describe a standard protocol for regenerating Arabidopsis plants in tissue culture, and for preparing and observing samples using confocal microscopy to study cells during regeneration.


Item Type:Book Section
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1007/978-1-62703-221-6_18DOIArticle
ORCID:
AuthorORCID
Meyerowitz, Elliot M.0000-0003-4798-5153
Additional Information:© 2013 Springer Science+Business Media. We thank Kazuhito Akama, Toshiro Ito, and Tatsuo Kakimoto for valuable information about tissue culture media formulations, Cory Tobin and Paul Tarr for information and technical suggestions about FM dye staining, and Carolyn Ohno for information about seeds sterilization. This work was supported by the US National Science Foundation (Grant IOS-0846192 to E.M.M.) and the Japan Society for the Promotion of Science (to K.S.).
Funders:
Funding AgencyGrant Number
NSFIOS-0846192
Japan Society for the Promotion of Science (JSPS)UNSPECIFIED
Subject Keywords:Arabidopsis thaliana , Plant regeneration , Tissue culture , Plant hormone , Callus formation , Shoot induction , Root induction
Series Name:Methods in Molecular Biology
Issue or Number:959
DOI:10.1007/978-1-62703-221-6_18
Record Number:CaltechAUTHORS:20130114-151324440
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20130114-151324440
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:36364
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:16 Jan 2013 19:19
Last Modified:09 Nov 2021 23:21

Repository Staff Only: item control page