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Evidence for two types of subunits in the bacterioferritin of Magnetospirillum magnetotacticum

Bertani, L. Elizabeth and Huang, Jerry S. and Weir, Barbara A. and Kirschvink, Joseph L. (1997) Evidence for two types of subunits in the bacterioferritin of Magnetospirillum magnetotacticum. Gene, 201 (1-2). pp. 31-36. ISSN 0378-1119. https://resolver.caltech.edu/CaltechAUTHORS:20130124-105456864

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Abstract

In order to investigate the role of bacterioferritin (Bfr) in the biomineralization of magnetite by microorganisms, we have cloned and sequenced the bfr genes from M. magnetotacticum. The organism has two bfr genes that overlap by one nucleotide. Both encode putative protein products of 18 kDa, the expected size for Bfr subunits, and show a strong similarity to other Bfr subunit proteins. By scanning the DNA sequence databases, we found that a limited number of other organisms, including N. gonorrhea, P. aeruginosa, and Synechocystis PCC6803, also have two bfr genes. When the sequences of a number of microbial Bfrs are compared with each other, they fall into two distinct types with the organisms mentioned above having one of each type. Differences in heme- and metal-binding sites and ferroxidase activities of the two types of subunits are discussed.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/S0378-1119(97)00424-1DOIUNSPECIFIED
http://www.sciencedirect.com/science/article/pii/S0378111997004241PublisherUNSPECIFIED
ORCID:
AuthorORCID
Kirschvink, Joseph L.0000-0001-9486-6689
Additional Information:© 1997 Elsevier Science B.V. Received 7 May 1997; accepted 3 July 1997; Received by Received by A.M. Campbell. We would like to thank David Mathog of the Sequence Analysis Facility for help with the molecular modelling. This work was supported by grant ES06652 from the National Institutes of Health and by a grant from the Undergraduate Biological Sciences Education Program of the Howard Hughes Medical Institute.
Funders:
Funding AgencyGrant Number
NIHES06652
Howard Hughes Medical Institute Undergraduate Biological Sciences Education ProgramUNSPECIFIED
Subject Keywords: Ferroxidase; Heme; Homology; Magnetite; Sequence
Issue or Number:1-2
Record Number:CaltechAUTHORS:20130124-105456864
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20130124-105456864
Official Citation:L. Elizabeth Bertani, Jerry S. Huang, Barbara A. Weir, Joseph L. Kirschvink, Evidence for two types of subunits in the bacterioferritin of Magnetospirillum magnetotacticum, Gene, Volume 201, Issues 1–2, 12 November 1997, Pages 31-36, ISSN 0378-1119, 10.1016/S0378-1119(97)00424-1. (http://www.sciencedirect.com/science/article/pii/S0378111997004241)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:36566
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:24 Jan 2013 19:26
Last Modified:03 Mar 2020 13:01

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