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Alternative mRNA Splicing of G Protein-Coupled Receptors

Markovic, Danijela (2013) Alternative mRNA Splicing of G Protein-Coupled Receptors. In: G Protein Coupled Receptor Structure. Methods in Enzymology. No.520. Academic Press , Oxford, pp. 323-335. ISBN 978-0-12-391861-1. https://resolver.caltech.edu/CaltechAUTHORS:20130128-112839673

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Abstract

Alternative mRNA splicing as an important mechanism for generating protein diversity has been implicated to affect 60–70% of human genes (Wang et al., 2008). The G protein-coupled receptors (GPCRs), the largest group of membrane receptors, have a broad distribution and function. Posttranslational modifications or/and association with regulatory proteins can govern cell- and tissue-specific pharmacological, signaling, and regulatory characteristics of GPCRs. However, increasing body of evidence suggests that alternative splicing of GPCRs is a mechanism that can modulate GPCRs’ function (Einstein et al., 2008). Many GPCRs have a potential to undergo alternative pre-mRNA splicing (∼ 50%). Due to a general lack of isoform-specific antibodies, GPCRs’ alternative splicing is mainly studied on mRNA level employing various PCR techniques. Functional characteristics (including signaling and structure) of alternatively spliced GPCRs are studied in overexpression system employing standard biochemical techniques. In this chapter, we will describe the methods for detection and quantification of alternatively spliced GPCR mRNA. As the experimental paradigm, we use type 1 corticotropin-releasing hormone receptor (CRH-R1).


Item Type:Book Section
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/B978-0-12-391861-1.00015-0DOIUNSPECIFIED
http://www.sciencedirect.com/science/article/pii/B9780123918611000150PublisherUNSPECIFIED
Additional Information:© 2013 Elsevier Inc. We like to thank Professors Dimitris Gramatopoulos and Hendrik Lehnert (Warwick Medical School) for financial support and Dr. Mei Gu (Warwick Medical School) for performing real-time PCRs.
Funders:
Funding AgencyGrant Number
Warwick Medical SchoolUNSPECIFIED
Subject Keywords:GPCR; Alternative splicing; mRNA; CRH-R1; Variants; Isoforms; PCR
Series Name:Methods in Enzymology
Issue or Number:520
DOI:10.1016/B978-0-12-391861-1.00015-0
Record Number:CaltechAUTHORS:20130128-112839673
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20130128-112839673
Official Citation:Danijela Markovic, Chapter Fifteen - Alternative mRNA Splicing of G Protein-Coupled Receptors, In: P. Michael Conn, Editor(s), Methods in Enzymology, Academic Press, 2013, Volume 520, Pages 323-335, ISSN 0076-6879, ISBN 9780123918611, 10.1016/B978-0-12-391861-1.00015-0. (http://www.sciencedirect.com/science/article/pii/B9780123918611000150)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:36625
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:28 Jan 2013 21:25
Last Modified:09 Nov 2021 23:23

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