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Catalysis of Dioxygen Reduction by Thermus thermophilus Strain HB27 Laccase on Ketjen Black Electrodes

Agbo, Peter and Heath, James R. and Gray, Harry B. (2013) Catalysis of Dioxygen Reduction by Thermus thermophilus Strain HB27 Laccase on Ketjen Black Electrodes. Journal of Physical Chemistry B, 117 (2). pp. 527-534. ISSN 1520-6106. PMCID PMC3548030. doi:10.1021/jp309759g.

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We present electrochemical analyses of the catalysis of dioxygen reduction by Thermus thermophilus strain HB27 laccase on ketjen black substrates. Our cathodes reliably produce 0.56 mA cm^(-2) at 0.0 V vs Ag|AgCl reference at 30 °C in air-saturated buffer, under conditions of nonlimiting O_(2) flux. We report the electrochemical activity of this laccase as a function of temperature, pH, time, and the efficiency of its conversion of dioxygen to water. We have measured the surface concentration of electrochemically active species, permitting the extraction of electron transfer rates at the enzyme-electrode interface: 1 s^(-1) for this process at zero driving force at 30 °C and a limiting rate of 23 s^(-1) at 240 mV overpotential at 50 °C.

Item Type:Article
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URLURL TypeDescription CentralArticle
Heath, James R.0000-0001-5356-4385
Gray, Harry B.0000-0002-7937-7876
Additional Information:© 2012 American Chemical Society. Received: October 2, 2012; Revised: November 7, 2012; Published: November 19, 2012. We thank James Mckone, Alec Durrell, and Bryan Stubbert for helpful discussions regarding electrochemistry and Kyle Lancaster for discussions regarding biochemistry. This research was funded by GCEP (Stanford), CSER (The Gordon and Betty Moore Foundation), and NIH (DK019038). Additional support was provided by a partial fellowship from the Institute for Collaborative Biotechnologies through grant W911NF-09- 0001 from the U.S. Army Research Office and the Department of Energy, Basic Energy Sciences (DE-FG03-01ER46175)(JRH PI).
Funding AgencyGrant Number
Global Climate and Energy Project (GCEP)UNSPECIFIED
Gordon and Betty Moore FoundationUNSPECIFIED
Army Research Office (ARO)W911NF-09-0001
Department of Energy (DOE)DE-FG03-01ER46175
Issue or Number:2
PubMed Central ID:PMC3548030
Record Number:CaltechAUTHORS:20130225-154105117
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Official Citation:Catalysis of Dioxygen Reduction by Thermus thermophilus Strain HB27 Laccase on Ketjen Black Electrodes Peter Agbo, James R. Heath, and Harry B. Gray The Journal of Physical Chemistry B2013117 (2), 527-534
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:37122
Deposited By: Nicole Ford
Deposited On:26 Feb 2013 00:04
Last Modified:09 Nov 2021 23:26

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