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Elk3 is essential for the progression from progenitor to definitive neural crest cell

Rogers, Crystal D. and Phillips, Jacquelyn L. and Bronner, Marianne E. (2013) Elk3 is essential for the progression from progenitor to definitive neural crest cell. Developmental Biology, 374 (2). pp. 255-263. ISSN 0012-1606. PMCID PMC3558539. doi:10.1016/j.ydbio.2012.12.009. https://resolver.caltech.edu/CaltechAUTHORS:20130321-131533041

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Abstract

Elk3/Net/Sap2 (here referred to as Elk3) is an Ets ternary complex transcriptional repressor known for its involvement in angiogenesis during embryonic development. Although Elk3 is expressed in various tissues, additional roles for the protein outside of vasculature development have yet to be reported. Here, we characterize the early spatiotemporal expression pattern of Elk3 in the avian embryo using whole mount in situ hybridization and quantitative RT-PCR and examine the effects of its loss of function on neural crest development. At early stages, Elk3 is expressed in the head folds, head mesenchyme, intersomitic vessels, and migratory cranial neural crest (NC) cells. Loss of the Elk3 protein results in the retention of Pax7+ precursors in the dorsal neural tube that fail to upregulate neural crest specifier genes, FoxD3, Sox10 and Snail2, resulting in embryos with severe migration defects. The results putatively place Elk3 downstream of neural plate border genes, but upstream of neural crest specifier genes in the neural crest gene regulatory network (NC-GRN), suggesting that it is critical for the progression from progenitor to definitive neural crest cell.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/j.ydbio.2012.12.009DOIArticle
http://www.sciencedirect.com/science/article/pii/S0012160612006756PublisherArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3558539/PubMed CentralArticle
ORCID:
AuthorORCID
Rogers, Crystal D.0000-0002-9549-1089
Bronner, Marianne E.0000-0003-4274-1862
Additional Information:© 2012 Elsevier Inc. Received 30 August 2012; Received in revised form; 13 December 2012; Accepted 14 December 2012; Available online 22 December 2012. We would like to thank Laura Gammill and Meghan Adams for previous identification of Elk3 and the Bronner lab for helpful discussions. This work was supported by NIH grant HD037105 to MEB, NIH minority supplement P01 HD037105 to CDR, and an Amgen summer fellowship and SURF fellowship to JLP.
Funders:
Funding AgencyGrant Number
NIHHD037105
NIHP01 HD037105
AmgenUNSPECIFIED
Caltech Summer Undergraduate Research Fellowship (SURF)UNSPECIFIED
Subject Keywords:Elk3; Neural crest specification; Progenitors; Pax7
Issue or Number:2
PubMed Central ID:PMC3558539
DOI:10.1016/j.ydbio.2012.12.009
Record Number:CaltechAUTHORS:20130321-131533041
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20130321-131533041
Official Citation:Crystal D. Rogers, Jacquelyn L. Phillips, Marianne E. Bronner, Elk3 is essential for the progression from progenitor to definitive neural crest cell, Developmental Biology, Volume 374, Issue 2, 15 February 2013, Pages 255-263, ISSN 0012-1606, 10.1016/j.ydbio.2012.12.009.
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:37588
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:09 Apr 2013 21:59
Last Modified:09 Nov 2021 23:30

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