CaltechAUTHORS
  A Caltech Library Service

Wide Field-of-View On-Chip Talbot Fluorescence Microscopy for Longitudinal Cell Culture Monitoring from within the Incubator

Han, Chao and Pang, Shuo and Bower, Danielle V. and Yiu, Patrick and Yang, Changhuei (2013) Wide Field-of-View On-Chip Talbot Fluorescence Microscopy for Longitudinal Cell Culture Monitoring from within the Incubator. Analytical Chemistry, 85 (4). pp. 2356-2360. ISSN 0003-2700. PMCID PMC3587116. https://resolver.caltech.edu/CaltechAUTHORS:20130325-083959903

[img] PDF - Accepted Version
See Usage Policy.

2906Kb
[img]
Preview
PDF - Supplemental Material
See Usage Policy.

478Kb
[img] Video (AVI) - Supplemental Material
See Usage Policy.

6Mb

Use this Persistent URL to link to this item: https://resolver.caltech.edu/CaltechAUTHORS:20130325-083959903

Abstract

Time-lapse or longitudinal fluorescence microscopy is broadly used in cell biology. However, current available time-lapse fluorescence microscopy systems are bulky and costly. The limited field-of-view (FOV) associated with the microscope objective necessitates mechanical scanning if a larger FOV is required. Here we demonstrate a wide FOV time-lapse fluorescence self-imaging Petri dish system, termed the Talbot Fluorescence ePetri, which addresses these issues. This system’s imaging is accomplished through the use of the Fluorescence Talbot Microscopy (FTM). By incorporating a microfluidic perfusion subsystem onto the platform, we can image cell cultures directly from within an incubator. Our prototype has a resolution limit of 1.2 μm and an FOV of 13 mm^2. As demonstration, we obtained time-lapse images of HeLa cells expressing H2B-eGFP. We also employed the system to analyze the cells’ dynamic response to an anticancer drug, camptothecin (CPT). This method can provide a compact and simple solution for automated fluorescence imaging of cell cultures in incubators.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1021/ac303356v DOIArticle
http://pubs.acs.org/doi/abs/10.1021/ac303356vPublisherArticle
http://pubs.acs.org/doi/suppl/10.1021/ac303356vPublisherSupporting Information
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3587116/PubMed CentralArticle
ORCID:
AuthorORCID
Bower, Danielle V.0000-0002-4469-4855
Yang, Changhuei0000-0001-8791-0354
Additional Information:© 2013 American Chemical Society. Received: November 20, 2012. Accepted: January 28, 2013. Published: January 28, 2013. We thank Dr. Scott Fraser (Biological Imaging Center, Caltech) for use of his lab facilities. This project is funded by National Institute of Health under Grant No. 1R01AI096226-01. D.V.B. is supported by NHLBI NRSA fellowship F30 HL110723.
Funders:
Funding AgencyGrant Number
NIH1R01AI096226-01
NIH Predoctoral FellowshipF30 HL110723
Issue or Number:4
PubMed Central ID:PMC3587116
Record Number:CaltechAUTHORS:20130325-083959903
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20130325-083959903
Official Citation:Wide Field-of-View On-Chip Talbot Fluorescence Microscopy for Longitudinal Cell Culture Monitoring from within the Incubator Chao Han, Shuo Pang, Danielle V. Bower, Patrick Yiu, and Changhuei Yang Analytical Chemistry201385 (4), 2356-2360
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:37603
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:25 Mar 2013 21:40
Last Modified:03 Oct 2019 04:49

Repository Staff Only: item control page