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Transcription Repressor HANABA TARANU Controls Flower Development by Integrating the Actions of Multiple Hormones, Floral Organ Specification Genes, and GATA3 Family Genes in Arabidopsis

Zhang, Xiaolan and Zhou, Yun and Ding, Lian and Wu, Zhigang and Liu, Renyi and Meyerowitz, Elliot M. (2013) Transcription Repressor HANABA TARANU Controls Flower Development by Integrating the Actions of Multiple Hormones, Floral Organ Specification Genes, and GATA3 Family Genes in Arabidopsis. Plant Cell, 25 (1). pp. 83-101. ISSN 1040-4651. PMCID PMC3584552. doi:10.1105/tpc.112.107854. https://resolver.caltech.edu/CaltechAUTHORS:20130409-101033645

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Abstract

Plant inflorescence meristems and floral meristems possess specific boundary domains that result in proper floral organ separation and specification. HANABA TARANU (HAN) encodes a boundary-expressed GATA3-type transcription factor that regulates shoot meristem organization and flower development in Arabidopsis thaliana, but the underlying mechanism remains unclear. Through time-course microarray analyses following transient overexpression of HAN, we found that HAN represses hundreds of genes, especially genes involved in hormone responses and floral organ specification. Transient overexpression of HAN also represses the expression of HAN and three other GATA3 family genes, HANL2 (HAN-LIKE 2), GNC (GATA, NITRATE-INDUCIBLE, CARBON-METABOLISM-INVOLVED), and GNL (GNC-LIKE), forming a negative regulatory feedback loop. Genetic analysis indicates that HAN and the three GATA3 family genes coordinately regulate floral development, and their expression patterns are partially overlapping. HAN can homodimerize and heterodimerize with the three proteins encoded by these genes, and HAN directly binds to its own promoter and the GNC promoter in vivo. These findings, along with the fact that constitutive overexpression of HAN produces an even stronger phenotype than the loss-of-function mutation, support the hypothesis that HAN functions as a key repressor that regulates floral development via regulatory networks involving genes in the GATA3 family, along with genes involved in hormone action and floral organ specification.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1105/tpc.112.107854DOIArticle
http://www.plantcell.org/content/25/1/83PublisherArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3584552/PubMed CentralArticle
ORCID:
AuthorORCID
Wu, Zhigang0000-0001-8959-2345
Meyerowitz, Elliot M.0000-0003-4798-5153
Additional Information:© 2013 American Society of Plant Biologists. Received November 29, 2012; revised November 29, 2012; accepted December 28, 2012; published January 18, 2013. Published online before print January 2013. We thank members of the Meyerowitz lab for the discussion and technique help. We thank Xuemei Chen and Xigang Liu for help with the in situ hybridization, Wolfgang Lukowitz for communicating unpublished information, Arnavaz Garda for technical assistance, Adrienne Roeder for sharing constructs before publication, and Adrienne Roeder, Kaoru Sugimoto, An Yan, and Zachary Nimchuk for critical reading and comments on the article. This work was supported by National Institutes of Health Grant 1R01 GM086639 to E.M.M., by the National Basic Research of China 973 Program 2012CB113900 and National Natural Science Foundation of China 31171399 to X.Z., and by a California Institute of Technology Gosney Postdoctoral Fellowship to Y.Z. Author Contributions: X.Z. and Y.Z. conceived and performed most of the experiments and wrote the article along with E.M.M. L.D. did the real-time PCR for data verification. Z.W. and R.L. performed the microarray data analyses.
Funders:
Funding AgencyGrant Number
NIH1R01 GM086639
National Basic Research of China 973 Program2012CB113900
National Natural Science Foundation of China31171399
Caltech Gosney FellowshipUNSPECIFIED
Issue or Number:1
PubMed Central ID:PMC3584552
DOI:10.1105/tpc.112.107854
Record Number:CaltechAUTHORS:20130409-101033645
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20130409-101033645
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:37820
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:15 Apr 2013 21:49
Last Modified:09 Nov 2021 23:31

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