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Dissimilatory arsenate and sulfate reduction in Desulfotomaculum auripigmentum sp. nov.

Newman, Dianne K. and Kennedy, Erin K. and Coates, John D. and Ahmann, Dianne and Ellis, Debra J. and Lovley, Derek R. and Morel, François M. M. (1997) Dissimilatory arsenate and sulfate reduction in Desulfotomaculum auripigmentum sp. nov. Archives of Microbiology, 168 (5). pp. 380-388. ISSN 0302-8933. doi:10.1007/s002030050512.

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A newly discovered arsenate-reducing bacterium, strain OREX-4, differed significantly from strains MIT-13 and SES-3, the previously described arsenate-reducing isolates, which grew on nitrate but not on sulfate. In contrast, strain OREX-4 did not respire nitrate but grew on lactate, with either arsenate or sulfate serving as the electron acceptor, and even preferred arsenate. Both arsenate and sulfate reduction were inhibited by molybdate. Strain OREX-4, a gram-positive bacterium with a hexagonal S-layer on its cell wall, metabolized compounds commonly used by sulfate reducers. Scorodite (FeAsO_42· H_2O) an arsenate-containing mineral, provided micromolar concentrations of arsenate that supported cell growth. Physiologically and phylogenetically, strain OREX-4 was far-removed from strains MIT-13 and SES-3: strain OREX-4 grew on different electron donors and electron acceptors, and fell within the gram-positive group of the Bacteria, whereas MIT-13 and SES-3 fell together in the ɛ-subdivision of the Proteobacteria. Together, these results suggest that organisms spread among diverse bacterial phyla can use arsenate as a terminal electron acceptor, and that dissimilatory arsenate reduction might occur in the sulfidogenic zone at arsenate concentrations of environmental interest. 16S rRNA sequence analysis indicated that strain OREX-4 is a new species of the genus Desulfotomaculum, and accordingly, the name Desulfotomaculum auripigmentum is proposed.

Item Type:Article
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URLURL TypeDescription
Newman, Dianne K.0000-0003-1647-1918
Morel, François M. M.0000-0002-6311-7826
Additional Information:© 1997 Springer-Verlag. Received: 22 October 1997; Accepted: 16 June 1997. We would like to thank P. Reilly (MIT, Cambridge, Mass., USA), T. Beveridge and D. Moyle (University of Guelph, Ontario, Canada) for TEM assistance, S. Lootens (Michigan State University Sequencing Facility, East Lansing, Mich., USA) for help with sequencing, and H. Spliethoff and R. Mason for providing us with surface sediments from Upper Mystic Lake. We are indebted to two anonymous reviewers for constructive criticism of earlier versions of this manuscript. This research was supported by the Office of Naval Research and the U.S. Environmental Protection Agency. D.K. Newman was supported by an NDSEG fellowship sponsored by the Department of Defense.
Funding AgencyGrant Number
Office of Naval Research (ONR)UNSPECIFIED
U.S. Environmental Protection Agency (EPA) UNSPECIFIED
Department of Defense (DoD) National Defense Science & Engineering Graduate Fellowship (NDSEG) ProgramUNSPECIFIED
Subject Keywords:Desulfotomaculum; Arsenate reduction; Sulfate reduction
Issue or Number:5
Record Number:CaltechAUTHORS:20130410-082430372
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Official Citation:Newman, D. K., E. K. Kennedy, et al. (1997). "Dissimilatory arsenate and sulfate reduction in Desulfotomaculum auripigmentum sp. nov." Archives of Microbiology 168(5): 380-388.
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:37843
Deposited By: Tony Diaz
Deposited On:12 Apr 2013 18:13
Last Modified:09 Nov 2021 23:31

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