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Microfluidic Digital PCR Enables Multigene Analysis of Individual Environmental Bacteria

Ottesen, Elizabeth A. and Hong, Jong Wook and Quake, Stephen R. and Leadbetter, Jared R. (2006) Microfluidic Digital PCR Enables Multigene Analysis of Individual Environmental Bacteria. Science, 314 (5804). pp. 1464-1467. ISSN 0036-8075. https://resolver.caltech.edu/CaltechAUTHORS:20130415-133930527

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Abstract

Gene inventory and metagenomic techniques have allowed rapid exploration of bacterial diversity and the potential physiologies present within microbial communities. However, it remains nontrivial to discover the identities of environmental bacteria carrying two or more genes of interest. We have used microfluidic digital polymerase chain reaction (PCR) to amplify and analyze multiple, different genes obtained from single bacterial cells harvested from nature. A gene encoding a key enzyme involved in the mutualistic symbiosis occurring between termites and their gut microbiota was used as an experimental hook to discover the previously unknown ribosomal RNA–based species identity of several symbionts. The ability to systematically identify bacteria carrying a particular gene and to link any two or more genes of interest to single species residing in complex ecosystems opens up new opportunities for research on the environment.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1126/science.1131370DOIArticle
ORCID:
AuthorORCID
Leadbetter, Jared R.0000-0002-7033-0844
Additional Information:© 2006 American Association for the Advancement of Science. Received 16 June 2006; accepted 30 October 2006. We thank M. Unger, A. Daridon, and L. Warren for advice and discussions. Supported by NIH grant 1RO1 HG002644-01A1, NIH National Research Service Award grant 5 T32 GM07616, an NIH Director's Pioneer Award, and NSF grant DEB-0321753. S.R.Q. is a founder, shareholder, and consultant for Fluidigm Corporation.
Funders:
Funding AgencyGrant Number
NIH1RO1 HG002644-01A1
NIH Predoctoral Fellowship5 T32 GM07616
NSFDEB-0321753
Issue or Number:5804
Record Number:CaltechAUTHORS:20130415-133930527
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20130415-133930527
Official Citation:Microfluidic Digital PCR Enables Multigene Analysis of Individual Environmental Bacteria Elizabeth A. Ottesen, Jong Wook Hong, Stephen R. Quake, and Jared R. Leadbetter Science 1 December 2006: 314 (5804), 1464-1467. [DOI:10.1126/science.1131370]
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:37944
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:15 Apr 2013 21:36
Last Modified:10 Jun 2020 23:07

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